Laboratoire de Valorisation, Analyses et Sécurité des Aliments, École Nationale d'Ingénieurs de Sfax, Route de Soukra, 3038 Sfax, Tunisia.
Laboratoire de Valorisation, Analyses et Sécurité des Aliments, École Nationale d'Ingénieurs de Sfax, Route de Soukra, 3038 Sfax, Tunisia.
Carbohydr Polym. 2021 Feb 1;253:117283. doi: 10.1016/j.carbpol.2020.117283. Epub 2020 Oct 23.
The effect of extraction procedures on chemical composition, structural, antitumor and anticoagulant properties of the sulphated polysaccharide 'ulvan' from the green seaweed Ulva lactuca were investigated. The structural features of ulvans were carried out by FTIR and by one- and two- dimensional H and C NMR spectroscopic. The ulvans were mainly composed of rhamnose, xylose, and uronic acid. Chemical and spectroscopic analyses demonstrated that ulvans were constituted of (1→4)-β-glucuronic acid, (1→3,4)-α-L-rhamnose-3-sulphate and (1→4)-α-xylose. The extraction procedures effect were observed in chemical structure, Mw and biological activities. Cytotoxic activity of enzymatic-chemical extract on cervical cancer cells (HeLa) (IC = 1000 μg/mL) was higher than on normal peripheral blood lymphocytes cells (PBL). Acid extracts promoted to reduce HeLa cells and to grow PBL cells. At high concentrations, acid extracts showed the highest APTT and TT clotting time. Antitumoral and anticoagulant activities of ulvans from Ulva lactuca promote their use as effective therapeutic agent.
研究了从绿藻石莼中提取的硫酸多糖“岩藻聚糖”的提取方法对其化学成分、结构、抗肿瘤和抗凝血特性的影响。通过傅里叶变换红外光谱（FTIR）和一维及二维氢碳核磁共振光谱（1H 和 13C NMR）对岩藻聚糖的结构特征进行了研究。岩藻聚糖主要由鼠李糖、木糖和糖醛酸组成。化学和光谱分析表明，岩藻聚糖由（1→4）-β-葡萄糖醛酸、（1→3,4）-α-L-鼠李糖-3-硫酸盐和（1→4）-α-木糖组成。提取方法对化学结构、Mw 和生物活性均有影响。酶化学提取物对宫颈癌（HeLa）细胞（IC = 1000 μg/mL）的细胞毒性活性高于对正常外周血淋巴细胞（PBL）细胞。酸提取物促进 HeLa 细胞减少和 PBL 细胞生长。在高浓度下，酸提取物显示出最高的 APTT 和 TT 凝血时间。石莼岩藻聚糖的抗肿瘤和抗凝血活性促进了其作为有效治疗剂的应用。
