Hughes Katherine, Scott Victoria H L, Blanck Maximilian, Barnett Timothy P, Spanner Kristiansen Jannie, Foote Alastair K
2 Horizon Discovery, Waterbeach, Cambridge, UK.
J Vet Diagn Invest. 2018 Mar;30(2):268-274. doi: 10.1177/1040638717745575. Epub 2017 Dec 3.
Hemangiosarcoma is an uncommon tumor in horses. We characterized 3 cases of equine renal hemangiosarcoma, focusing on clinical and pathologic features, and describe occurrence of the epithelioid variant of hemangiosarcoma in one of these cases. Nuclear expression of phosphorylated STAT3 (pSTAT3) was assessed to analyze potential inappropriate STAT3 activation as a component of tumor pathogenesis. Clinical signs in the 3 horses included insidious weight loss, followed in one case by serosanguineous nasal discharge and terminal epistaxis, and nonspecific signs of abdominal pain. Two of the hemangiosarcomas had a classical histopathologic appearance; in the other, neoplastic cells were polygonal and were arranged in densely packed sheets, resembling the epithelioid variant. Cross-reactivity of a pSTAT3 antibody was established by demonstration of pSTAT3 expression in the epithelium of glabrous skin by immunoblotting and immunohistochemistry. In the epithelioid hemangiosarcoma, ~40% of neoplastic cells exhibited nuclear pSTAT3 expression, but in the other 2 cases, expression was weak and variable in the neoplastic population, although stromal cell pSTAT3 activity was evident in pulmonary metastases in one case.
血管肉瘤在马中是一种罕见的肿瘤。我们对3例马肾血管肉瘤进行了特征描述，重点关注临床和病理特征，并描述了其中1例血管肉瘤上皮样变体的发生情况。评估磷酸化STAT3（pSTAT3）的核表达，以分析作为肿瘤发病机制一部分的潜在不适当的STAT3激活。3匹马的临床症状包括隐匿性体重减轻，其中1例随后出现浆液性鼻漏和终末期鼻出血，以及腹痛的非特异性症状。其中2例血管肉瘤具有典型的组织病理学表现；另一例中，肿瘤细胞呈多边形，排列成密集的片状，类似于上皮样变体。通过免疫印迹和免疫组织化学在无毛皮肤上皮中证实pSTAT3表达，从而确定了pSTAT3抗体的交叉反应性。在上皮样血管肉瘤中，约40%的肿瘤细胞呈现核pSTAT3表达，但在其他2例中，肿瘤细胞群体中的表达较弱且变化不定，尽管在1例的肺转移灶中基质细胞pSTAT3活性明显。
