Knežević Aleksandar, Stajić Mirjana, Živković Lada, Milovanović Ivan, Spremo-Potparević Biljana, Vukojević Jelena
Faculty of Biology, University of Belgrade, Belgrade, Serbia.
Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
Int J Med Mushrooms. 2017;19(6):509-520. doi: 10.1615/IntJMedMushrooms.v19.i6.30.
This study aimed to evaluate the antifungal, antioxidative, and genoprotective potentials of various extracts of Daedaleopsis confragosa fruiting bodies and mycelia. The extracts exhibited weak fungistatic and an absence of any fungicidal activities against 7 important animal and human pathogens. Although mycelial extracts, especially the hot water extract, were more effective antifungal agents than basidiocarp extracts, they were significantly weaker than the commercial antimycotic ketoconazole. The tested extracts reduced DPPH radicals to various extents, depending on the material used for extraction and the solvent. The mycelial hot water extract was the best DPPH radical scavenger (half-maximal effective concentration, 3.42 mg mL-1), whereas the 96` ethanol extract of basidiocarps was the weakest one (half-maximal effective concentration, 5.36 mg mL-1). Phenols were the main carriers of activity, and their proportions in the tested extract mainly depended on the type and concentration of the solvent; the effect of the material used for extraction was negligible. The largest amount of phenols was detected in the 70% ethanol extract of basidiocarps (126.40 μg gallic acid equivalents mg-1) and the lowest in the basidiocarp methanol extract (19.93 μg gallic acid equivalents mg-1). Aqueous extracts of basidiocarp and mycelial extracts were not genotoxic agents but exhibited significant protective activity against hydrogen peroxide-induced DNA damage during use in leukocytes' pre- and post-treatments.
本研究旨在评估毛云芝子实体和菌丝体的各种提取物的抗真菌、抗氧化和基因保护潜力。这些提取物对7种重要的动物和人类病原体表现出微弱的抑菌作用，且无任何杀菌活性。虽然菌丝体提取物，尤其是热水提取物，比子实体提取物是更有效的抗真菌剂，但它们明显弱于市售抗真菌药酮康唑。根据用于提取的材料和溶剂的不同，受试提取物在不同程度上还原了DPPH自由基。菌丝体热水提取物是最佳的DPPH自由基清除剂（半数有效浓度为3.42 mg mL-1），而子实体的96%乙醇提取物是最弱的一种（半数有效浓度为5.36 mg mL-1）。酚类是主要的活性载体，它们在受试提取物中的比例主要取决于溶剂的类型和浓度；用于提取的材料的影响可忽略不计。在子实体的70%乙醇提取物中检测到的酚类含量最高（126.40 μg没食子酸当量mg-1），而在子实体甲醇提取物中最低（19.93 μg没食子酸当量mg-1）。子实体水提取物和菌丝体提取物不是基因毒性剂，但在白细胞预处理和后处理过程中，对过氧化氢诱导的DNA损伤表现出显著的保护活性。
