六种精油对洋葱伯克霍尔德氏菌复合体的抗菌活性：作用机制的研究。
Antimicrobial activity of six essential oils against Burkholderia cepacia complex: insights into mechanism(s) of action.
机构信息
Department of Biology, University of Florence, Sesto Fiorentino I-50019, Florence, Italy.
Department of Experimental & Clinical Medicine, University of Florence, I-50134, Florence, Italy.
出版信息
Future Microbiol. 2018 Jan;13:59-67. doi: 10.2217/fmb-2017-0121. Epub 2017 Dec 4.
AIM
To investigate the activity and mechanisms of action of six essential oils (EOs) against Burkholderia cepacia complex, opportunistic human pathogens highly resistant to antibiotics.
MATERIALS & METHODS: Minimal inhibitory concentration of EOs alone, plus antibiotics or efflux pump inhibitors was determined.
RESULTS
Origanum vulgare, Thymus vulgaris and Eugenia caryophyllata EOs resulted to be more active than the other EOs. EOs did not enhance antibiotic activity against the model strain B. cenocepacia J2315. EOs resulted more active in the presence of an efflux pump inhibitor acting on Resistance-Nodulation Cell Division efflux pumps and against B. cenocepacia J2315 Resistance-Nodulation Cell Division knocked-out mutants.
CONCLUSION
EOs showed intracellular mechanisms of action and, thus, the efflux pumps inhibitor addition could boost their activity.
目的
研究六种精油（EOs）对洋葱伯克霍尔德氏菌复合体（一种对多种抗生素具有高度耐药性的机会性病原体）的活性和作用机制。
材料与方法
单独测定了 EOs 的最小抑菌浓度，以及 EOs 与抗生素或外排泵抑制剂的联合作用。
结果
牛至、普通麝香草和丁香精油比其他 EOs 更具活性。EOs 并没有增强抗生素对模式菌株 B. cenocepacia J2315 的活性。在作用于耐药-结节-分裂（Resistance-Nodulation Cell Division）外排泵的外排泵抑制剂存在的情况下，EOs 的活性更高，并且对 B. cenocepacia J2315 Resistance-Nodulation Cell Division 敲除突变体也更有效。
结论
EOs 显示出细胞内作用机制，因此，添加外排泵抑制剂可以增强其活性。
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