Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730030, China; Gansu Tech Innovation Center of Animal Cell, Biomedical Research Center, Northwest Minzu University, Lanzhou 730030, China.
Gene. 2022 Aug 5;834:146598. doi: 10.1016/j.gene.2022.146598. Epub 2022 May 20.
Lysine demethylase 3B (KDM3B), a candidate gene associated with bone formation and growth, and differentiation of osteoblast, might affect the animal growth traits. Herein, the insertion/deletion (InDel) of the KDM3B gene was quickly detected in 882 sheep from five breeds using the mathematical expectation (ME) method. The results showed that there were two genotypes of 7-bp variation in KDM3B, including II (insertion/insertion) and ID (insertion/deletion), and the frequency of two genotypes varied among the five sheep breeds. Association analysis results demonstrated that the 7-bp indel was significantly associated with chest depth of LFT sheep (P = 0.012), and body weight (P = 0.006), body height (P = 0.030), chest depth (P = 0.043), chest circumference (P = 0.016), abdominal width (P = 0.035) and height at hip cross (P = 0.022) in LXBH sheep. Moreover, II genotype was the predominant genotype with excellent consistency in sheep growth traits (P < 0.05). Collectively, the above results suggest that this locus can be used as an effective molecular marker to improve the sheep growth traits and provide a scientific basis for the development of sheep breeding.
赖氨酸去甲基酶 3B（KDM3B）是一个与骨形成和生长以及成骨细胞分化相关的候选基因，可能会影响动物的生长性状。在此，使用数学期望（ME）方法快速检测了来自五个品种的 882 只绵羊中的 KDM3B 基因的插入/缺失（InDel）。结果表明，KDM3B 存在 7-bp 变异的两种基因型，包括 II（插入/插入）和 ID（插入/缺失），两种基因型在五个绵羊品种中的频率不同。关联分析结果表明，7-bp 插入缺失与 LFT 绵羊的胸深（P=0.012）、体重（P=0.006）、体高（P=0.030）、胸深（P=0.043）、胸围（P=0.016）、腹宽（P=0.035）和髋骨交叉高度（P=0.022）显著相关。此外，II 基因型是主要的基因型，在绵羊生长性状上具有良好的一致性（P<0.05）。综上所述，该位点可作为有效分子标记，用于提高绵羊的生长性状，为绵羊育种的发展提供科学依据。
