Lall Namrita, Kishore Navneet, Fibrich Bianca, Lambrechts Isa Anina
Department of Plant and Soil Sciences, University of Pretoria, Pretoria 0002, South Africa.
Pharmacogn Mag. 2017 Oct-Dec;13(52):583-589. doi: 10.4103/pm.pm_145_17. Epub 2017 Nov 13.
(MA) is a plant traditionally used in South Africa to treat various diseases.
The ethanolic extract of MA, was used for and studies to determine its elastase inhibitory activity.
MA and its isolated compound, myrsinoside B, were tested for their elastase inhibitory activity. The MA extract was also evaluated for mutagenicity using two strains of (TA 98 and TA 100), microbial count, metal analysis, and stability. studies included irritancy and wrinkle reduction trials using Visioscan and Visioface.
The leaf extract showed good elastase inhibition with a 50% inhibitory concentration (IC) of 28.04 μg/ml. Myrsinoside B inhibited the elastase enzyme at an IC of 4.68 ± 0.34 μg/ml. No colony growth observed during mutagenicity studies and it was concluded that MA ethanolic extract is a nonmutagen. MA extract was found to be a nonirritant during the patch test clinical trial. MA was found to contain negligible amounts of microorganisms and heavy metals. Gel cream containing MA crude extract was found to be stable for 2 years when kept at temperatures below 30°C. In clinical trials (), it was found that the test product containing 5% ethanolic extract of MA was effective in reducing wrinkles after application 2 times a day for 14 days and 28 days compared to the placebo aqueous cream.
MA is effective in reducing the appearance of wrinkles.
This is a first time report of the elastase inhibitory potential of and myrsinoside B and the anti-wrinkle potential of ethanolic extract effectively inhibited the elastase enzyme was effective in studies to reduce the appearance of wrinkles after 14 days. 4-NQO: 4-nitroquinoline, D14-BL: Baseline to day fourteen, D28-BL: Baseline to day twenty-eight, CFU: Colony forming units, IC: 50% inhibitory concentration, MA: , MOU: Measurement of uncertainty, NaCl: Sodium chloride, NaH PO.HO: Sodium phosphate monobasic monohydrate, SEM: Standard error of the mean, TA 98: strain 98, TA 100: strain 100, TLC: Thin layer chromatography, TMA: Total microbial activity, XVB salt: Vogel-Bonner medium E.
（MA）是一种传统上在南非用于治疗各种疾病的植物。
MA的乙醇提取物用于[具体用途1]和[具体用途2]研究，以确定其弹性蛋白酶抑制活性。
对MA及其分离出的化合物杨梅苷B进行弹性蛋白酶抑制活性测试。还使用两种[细菌菌株名称]（TA 98和TA 100）对MA提取物进行致突变性评估、微生物计数、金属分析和稳定性研究。[具体研究]包括使用Visioscan和Visioface进行刺激性和减少皱纹试验。
叶提取物显示出良好的弹性蛋白酶抑制作用，50%抑制浓度（IC）为28.04μg/ml。杨梅苷B在IC为4.68±0.34μg/ml时抑制弹性蛋白酶。在致突变性研究中未观察到菌落生长，得出结论MA乙醇提取物无致突变性。在斑贴试验临床试验中发现MA提取物无刺激性。发现MA含有极少量的微生物和重金属。含MA粗提取物的凝胶霜在低于30°C的温度下保存2年时是稳定的。在临床试验（[具体临床试验相关信息]）中，发现含有5%MA乙醇提取物的测试产品与安慰剂水性乳膏相比，每天涂抹2次，持续14天和28天后，在减少皱纹方面有效。
MA在减少皱纹外观方面有效。
这是首次报道[植物名称]和杨梅苷B的弹性蛋白酶抑制潜力以及[植物名称]乙醇提取物的抗皱潜力，有效抑制弹性蛋白酶的提取物在[具体研究]中14天后对减少皱纹外观有效。4-NQO：4-硝基喹啉，D14-BL：从基线到第14天，D28-BL：从基线到第28天，CFU：菌落形成单位，IC：50%抑制浓度，MA：[植物名称]，MOU：不确定度测量，NaCl：氯化钠，NaH₂PO₄·H₂O：磷酸二氢钠一水合物，SEM：均值的标准误差，TA 98：[细菌菌株98]，TA 100：[细菌菌株100]，TLC：薄层色谱法，TMA：总微生物活性，XVB盐：Vogel-Bonner培养基E 。
