Tsukahara Kazue, Nakagawa Hidemi, Moriwaki Shigeru, Takema Yoshinori, Fujimura Tsutomu, Imokawa Genji
Department of Dermatology, Jichi Medical School, and Kao Biological Science Laboratories, Tochigi, Japan.
Int J Dermatol. 2006 Apr;45(4):460-8. doi: 10.1111/j.1365-4632.2006.02557.x.
Previously, we have demonstrated that fibroblast-derived elastase plays an essential role in the increased three-dimensional tortuosity of elastic fibers, contributing to the loss of skin elasticity in UV-B-exposed skin. This decrease in skin elasticity is closely associated with the formation of wrinkles induced by UV exposure.
To further clarify the role of elastase in the formation of wrinkles induced by UV exposure, we assessed the effects of an extract of Zingiber officinale (L.) Rose (which inhibits fibroblast-derived elastase) on the wrinkle formation induced by chronic UV-B irradiation.
Topical application of an extract of Zingiber officinale (L.) Rose to rat or hairless mouse skin significantly inhibited the wrinkle formation induced by chronic UV-B irradiation at a suberythemal dose, which was accompanied by a significant prevention of the decrease in skin elasticity in both types of animal skin. In the rat hind limb skin, consistent with the inhibition of reduced skin elasticity, wrinkle prevention occurred concomitantly with a significant decrease in the curling and three-dimensional tortuosity of dermal elastic fibers.
Our results indicate that herbal extracts with an ability to inhibit fibroblast-derived elastase may prove to be effective as anti-wrinkling agents, confirming the important role of elastase in UV-B-induced wrinkle formation.
此前，我们已经证明成纤维细胞衍生的弹性蛋白酶在弹性纤维三维曲折度增加中起重要作用，这导致了紫外线B照射皮肤中皮肤弹性的丧失。皮肤弹性的这种降低与紫外线照射引起的皱纹形成密切相关。
为了进一步阐明弹性蛋白酶在紫外线照射诱导皱纹形成中的作用，我们评估了姜（抑制成纤维细胞衍生的弹性蛋白酶）提取物对慢性紫外线B照射诱导的皱纹形成的影响。
将姜提取物局部应用于大鼠或无毛小鼠皮肤，在亚红斑剂量下可显著抑制慢性紫外线B照射诱导的皱纹形成，同时两种动物皮肤的皮肤弹性降低均得到显著预防。在大鼠后肢皮肤中，与皮肤弹性降低的抑制一致，皱纹预防伴随着真皮弹性纤维卷曲和三维曲折度的显著降低。
我们的结果表明，具有抑制成纤维细胞衍生弹性蛋白酶能力的草药提取物可能被证明是有效的抗皱剂，证实了弹性蛋白酶在紫外线B诱导的皱纹形成中的重要作用。
