Superoxide Scavenging and Antiglycation Activity of Rhinacanthins-rich Extract Obtained from the Leaves of .

BACKGROUND

Oxidative stress and nonenzymatic protein glycation lead to serious diabetic complications that increase the risk of mortality. leaf crude extracts are previously reported for their antidiabetic, antiglycation, and antioxidant potential.

OBJECTIVE

The present study was performed to prepare a standardized rhinacanthins-rich extract (RRE) and evaluate its superoxide scavenging and antiglycation effects as compared to its marker compounds, namely, rhinacanthin-C (RC), rhinacanthin-D (RD), and rhinacanthin-N (RN).

MATERIALS AND METHODS

RRE was obtained by microwave-assisted green extraction along with a simple step of fractionation using Amberlite column. RC, RD, and RN were isolated from the RRE using silica gel column chromatography. Superoxide scavenging activity was performed by cyclic voltammetry, and fructose-mediated human serum albumin glycation model was used for antiglycation activity. studies were conducted to identify the structure-activity relationships of rhinacanthins.

RESULTS

On the basis of kinetic measurements, RRE exhibited the most potent antioxidant activity EC mechanism, with a 50% inhibitory concentration (IC) value of 8.0 μg/mL, antioxidant capacity of 39439 M, and binding constant of 45709 M. Antiglycation assay showed that RRE exhibited almost equivalent glycation inhibitory effect to that of RC, with IC values of 39.7 and 37.3 μg/mL, respectively, but higher than that of RD (IC of 50.4 μg/mL), RN (IC of 89.5 μg/mL), as well as the positive control, rutin (IC of 41.5 μg/mL).

CONCLUSIONS

The potent superoxide scavenging and albumin glycation inhibitory effect of RRE rationalized its therapeutic application in various chronic diseases, especially in the complications of diabetes.

SUMMARY

Rhinacanthins-rich extract (RRE) exhibited potent superoxide scavenging activityRRE and rhinacanthin-C showed remarkable and comparable antiglycation effectRhinacanthins exhibited antiglycation activity by masking specific residues of albumin. RRE: Rhinacanthins-rich extract; RC: Rhinacanthin-C; RD: Rhinacanthin-D; RN: Rhinacanthin-N; IC: 50% inhibitory concentration; : Antioxidant activity coefficient; : Binding constant; EC: Reversible electron transfer followed by an irreversible chemical reaction; DM: Diabetes mellitus; AGEPs: Advanced glycation end products; NMR: Nuclear magnetic resonance; HPLC: High-performance liquid chromatography; CV: Cyclic voltammetry; DMSO: Dimethyl sulfoxide; Ipa: Anodic peak current; Ipc: Cathodic peak current; HSA: Human serum albumin; MOE: Molecular operating environment; PASSonline: Online prediction of activity spectra for substances.