Subramanya M D, Pai Sandeep R, Ankad Gireesh M, Hegde Harsha V, Roy Subarna, Hoti S L
Department of Dravyaguna, BMK Ayurveda Mahavidyalaya, Belagavi, Karnataka, India.
Department of Biotechnology, Amity Institute of Biotechnology, Amity University Mumbai, Maharastra, India.
Ayu. 2016 Apr-Jun;37(2):135-139. doi: 10.4103/ayu.AYU_49_16.
L. is a medicinally important genus widely used in conventional systems of medicine in India.
The present study aims toward simultaneous determination of two bioactive compounds vasicine and vasicinone in root extracts of eight spp. from Western Ghats, India.
Determination of vasicine and vasicinone was undertaken in methanolic root extracts (10% w/v) of , , , , , , and by high performance liquid chromatography (HPLC) method. The standards were prepared with the concentration of mg/mL. Data were expressed as mean values of three reading and relative standard deviations. The separation was achieved on a Waters, Nova-Pack, C18 (250 mm × 4.6 mm, 5 μ) column, with acetonitrile - 0.1 M phosphate buffer-glacial acetic acid (15: 85: 1, v/v/v) as solvent system at a flow-rate of 1.0 mL/min. The effluent was monitored using ultraviolet detection at a wavelength of 300 nm.
Both calibration curves of standard showed good linear regression ( > 0.994). The limit of detection and the limit of quantification for vasicine was 0.110 and 0.333 μg/mL and for vasicinone was 0.059 and 0.179 μg/mL respectively. The vasicine content was highest in (9.891 ± 0.495 μg/100 mg) and vasicinone content was rich in (33.013 ± 1.651 μg/100 mg.) The content of vasicinone was higher than vasicine.
HPLC method provides simple, accurate, and reproducible quantitative analysis for simultaneous determination of vasicine and vasicinone. Among the selected species, and were found to be rich in the vasicine and vasicinone contents, respectively.
L. 是一个具有重要药用价值的属，在印度传统医学体系中被广泛使用。
本研究旨在同时测定来自印度西高止山脉的八个物种根提取物中的两种生物活性化合物鸭嘴花碱和去甲鸭嘴花碱。
采用高效液相色谱（HPLC）法测定了、、、、、、和的甲醇根提取物（10% w/v）中的鸭嘴花碱和去甲鸭嘴花碱。标准品的浓度为mg/mL。数据以三次读数的平均值和相对标准偏差表示。分离在 Waters Nova-Pack C18（250 mm × 4.6 mm，5 μ）柱上进行，以乙腈 - 0.1 M磷酸盐缓冲液 - 冰醋酸（15: 85: 1，v/v/v）作为溶剂系统，流速为1.0 mL/min。流出物在300 nm波长下用紫外检测进行监测。
两种标准品的校准曲线均显示出良好的线性回归（> 0.994）。鸭嘴花碱的检测限和定量限分别为0.110和0.333 μg/mL，去甲鸭嘴花碱的检测限和定量限分别为0.059和0.179 μg/mL。鸭嘴花碱含量在中最高（9.891 ± 0.495 μg/100 mg），去甲鸭嘴花碱含量在中最高（33.013 ± 1.651 μg/100 mg）。去甲鸭嘴花碱的含量高于鸭嘴花碱。
HPLC法为同时测定鸭嘴花碱和去甲鸭嘴花碱提供了简单、准确且可重复的定量分析方法。在所选择的物种中，和分别被发现富含鸭嘴花碱和去甲鸭嘴花碱。
