Subramanya M D, Pai Sandeep R, Upadhya Vinayak, Ankad Gireesh M, Bhagwat Shalini S, Hegde Harsha V
Department of Dravya Guna Vidnyana, Karnatak Lingayat Education University, BMK Ayurveda Mahavidyalaya, Belgaum, Karnataka, India.
Plant Biotechnology and Tissue Culture Division, Regional Medical Research Centre, Indian Council of Medical Research, Belgaum, Karnataka, India.
J Ayurveda Integr Med. 2015 Jan-Mar;6(1):24-8. doi: 10.4103/0975-9476.146544.
Sida L., is a medicinally important genus, the species of which are widely used in traditional systems of medicine in India. Pharmacologically, roots are known for anti-tumor, anti-HIV, hepatoprotective, and many other properties. Phenolic antioxidants help in reducing oxidative stress occurring during treatment of such diseases.
The study aimed to evaluate and compare polyphenol contents and antioxidant properties of eight selected species of Sida from Western Ghats, India.
Methanolic root extracts (10% w/v) of Sida species, viz., S. acuta, S. cordata, S. cordifolia, S. indica, S. mysorensis, S. retusa, S. rhombifolia, and S. spinosa were analyzed.
Sida cordifolia possessed highest total phenolic content (TPC: 1.92 ± 0.10 mg Caffeic Acid Equivalent/g and 2.13 ± 0.11 mg Tannic Acid Equivalant/g), total flavonoid content (TF: 2.60 ± 0.13 mg Quercetin Equivalent/g) and also possessed highest antioxidant activities in 2,2-diphenylpicrylhydrazyl (DPPH) radical scavenging (51.31 ± 2.57% Radical Scavenging Activity, (RSA); Trolox Equivalent Antioxidant Capacity: 566.25 ± 28.31μM; Ascorbic acid Equivalent Antioxidant Capacity: 477.80 ± 23.89 μM) and Ferric Reducing Antioxidant Power assays (TEAC: 590.67 ± 29.53 μM; AEAC: 600.67 ± 30.03 μM). Unlike DPPH and Ferric Reducing Antioxidant Power (FRAP) activity, 2, 2'-Azinobis (3-ethyl Benzo Thiazoline-6-Sulfonic acid) ABTS(+) antioxidant activity was highest in S. indica (TEAC: 878.44 ± 43.92 μM; AEAC 968.44 ± 48.42 μM). It was significant to note that values of AEAC (μM) for all the antioxidant activities analyzed were higher than that of TEAC.
The high contents of phenolic compounds in the root extracts of selected Sida species have direct correlation with their antioxidant properties. Conclusively, roots of S. cordifolia can be considered as the potential source of polyphenols and antioxidants.
黄花稔属是一个具有重要药用价值的属，其物种在印度传统医学体系中被广泛应用。从药理学角度来看，其根具有抗肿瘤、抗艾滋病毒、保肝等多种特性。酚类抗氧化剂有助于减轻此类疾病治疗过程中产生的氧化应激。
本研究旨在评估和比较印度西高止山脉地区八种黄花稔属植物的多酚含量和抗氧化特性。
分析了黄花稔属植物的甲醇根提取物（10% w/v），即尖齿黄花稔、心叶黄花稔、线叶黄花稔、印度黄花稔、迈索尔黄花稔、平卧黄花稔、菱叶黄花稔和刺黄花稔。
线叶黄花稔的总酚含量最高（总酚含量：1.92±0.10毫克咖啡酸当量/克和2.13±0.11毫克单宁酸当量/克），总黄酮含量（总黄酮：2.60±0.13毫克槲皮素当量/克）也最高，并且在2,2 - 二苯基苦味酰基自由基清除（DPPH）实验（51.31±2.57%自由基清除活性，（RSA）；Trolox当量抗氧化能力：566.25±28.31μM；抗坏血酸当量抗氧化能力：477.80±23.89μM）和铁还原抗氧化能力实验中具有最高的抗氧化活性（TEAC：590.67±29.53μM；AEAC：600.67±30.03μM）。与DPPH和铁还原抗氧化能力（FRAP）活性不同，2,2'-联氮 - 双 -（3 - 乙基苯并噻唑啉 - 6 - 磺酸）ABTS(+)抗氧化活性在印度黄花稔中最高（TEAC：878.44±43.92μM；AEAC 968.44±48.42μM）。值得注意的是，所有分析的抗氧化活性的AEAC（μM）值均高于TEAC。
所选黄花稔属植物根提取物中酚类化合物的高含量与其抗氧化特性直接相关。总之，线叶黄花稔的根可被视为多酚和抗氧化剂的潜在来源。
