Tang Yuanman, Liu Qiuping, Liu Ying, Zhang Linli, Ding Wei
College of Plant Protection, Southwest University, Chongqing, China.
Front Plant Sci. 2017 Nov 16;8:1963. doi: 10.3389/fpls.2017.01963. eCollection 2017.
Various classes of plant pathogenesis-related proteins have been identified in the past several decades. PR-Q, a member of the PR3 family encoding chitinases, has played an important role in regulating plant resistance and preventing pathogen infection. In this paper, we functionally characterized in tobacco plants and found that the overexpression of in tobacco Yunyan87 resulted in higher resistance to inoculation. Surprisingly, overexpression of led to the activation of many defense-related genes, such as salicylic acid (SA)-responsive genes , and , JA-responsive gene and ET production-associated genes and . Consistent with the role of in multiple stress responses, transcripts were induced by the exogenous hormones SA, ethylene and methyl jasmonate, which could enhance the resistance of tobacco to . Collectively, our results suggested that overexpression led to the up-regulation of defense-related genes and enhanced plant resistance to infection.
在过去几十年中，已鉴定出各类植物病程相关蛋白。PR-Q是编码几丁质酶的PR3家族成员，在调节植物抗性和预防病原体感染方面发挥了重要作用。在本文中，我们对烟草植株进行了功能特性分析，发现PR-Q在烟草云烟87中的过表达导致对赤星病菌接种具有更高的抗性。令人惊讶的是，PR-Q的过表达导致许多防御相关基因的激活，如水杨酸（SA）响应基因PR1、PR2和PR5，茉莉酸响应基因PDF1.2以及乙烯产生相关基因ACS和ETR。与PR-Q在多种胁迫反应中的作用一致，PR-Q转录本受外源激素SA、乙烯和茉莉酸甲酯诱导，这可以增强烟草对赤星病菌的抗性。总体而言，我们的结果表明PR-Q过表达导致防御相关基因上调并增强了植物对赤星病菌感染的抗性。
