Guo Wei-Li, Yang He-Lian, Zhao Jin-Peng, Bian Shi-Jie, Guo Yan-Yan, Chen Xue-Jin, Li Xin-Zheng
School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang, China.
Henan Province Engineering Research Center of Horticultural Plant Resource Utilization and Germplasm Enhancement, Xinxiang, China.
Front Genet. 2023 Aug 1;14:1168138. doi: 10.3389/fgene.2023.1168138. eCollection 2023.
Pumpkin ( Duch.) productivity is severely hindered by powdery mildew (PM) worldwide. The causative agent of pumpkin PM is , a biotrophic fungus. Pathogenesis-related protein 1 (PR1) homolog was previously identified from transcriptomic analysis of a PM-resistant pumpkin. Here, we investigated the effects of gene from pumpkin for resistance to PM. Subcellular localization assay revealed that is a cytoplasmic protein in plants. The expression of gene was strongly induced by inoculation at 48 h and exogenous ethylene (ET), jasmonic acid (JA) and NaCl treatments, but repressed by HO and salicylic acid (SA) treatments. Visual disease symptoms, histological observations of fungal growth and host cell death, and accumulation of HO in transgenic tobacco plants indicated that overexpression significantly enhanced the resistance to compared to wild type plants during PM pathogens infection, possibly due to inducing cell death and HO accumulation near infected sites. The expression of was significantly induced in transgenic tobacco plants in response to , suggesting that overexpression positively modulates the resistance to PM via the SA signaling pathway. These findings indicate that is a defense response gene in and can be exploited to develop disease-resistant crop varieties.
在全球范围内，南瓜（西葫芦）的产量受到白粉病（PM）的严重阻碍。南瓜白粉病的病原体是一种活体营养型真菌。先前通过对一种抗白粉病南瓜的转录组分析鉴定出了病程相关蛋白1（PR1）同源物。在此，我们研究了南瓜中 基因对抵抗白粉病的作用。亚细胞定位分析表明， 在植物中是一种细胞质蛋白。 基因的表达在接种48小时时以及外源乙烯（ET）、茉莉酸（JA）和氯化钠处理后被强烈诱导，但在过氧化氢（HO）和水杨酸（SA）处理下受到抑制。转基因烟草植株中的视觉病害症状、真菌生长和宿主细胞死亡的组织学观察以及HO的积累表明，与野生型植株相比，在白粉病病原体感染期间， 基因的过表达显著增强了对 的抗性，这可能是由于诱导了感染部位附近的细胞死亡和HO积累。 的表达在转基因烟草植株中响应 被显著诱导，表明 基因的过表达通过水杨酸信号通路正向调节对白粉病的抗性。这些发现表明， 在 中是一个防御反应基因，可用于培育抗病作物品种。
