Liu Ying, Tang Yuanman, Tan Xi, Ding Wei
College of Plant Protection, Southwest University, Chongqing 400715, China.
Int J Mol Sci. 2021 May 24;22(11):5507. doi: 10.3390/ijms22115507.
E3 ubiquitin ligases, the most important part of the ubiquitination process, participate in various processes of plant immune response. RBR E3 ligase is one of the E3 family members, but its functions in plant immunity are still little known. NtRNF217 is a RBR E3 ligase in tobacco based on the sequence analysis. To assess roles of in tobacco responding to , overexpression experiments in (Yunyan 87, a susceptible cultivar) were performed. The results illuminated that -overexpressed tobacco significantly reduced multiplication of and inhibited the development of disease symptoms compared with wild-type plants. The accumulation of HO and O in -OE plants was significantly higher than that in WT-Yunyan87 plants after pathogen inoculation. The activities of CAT and SOD also increased rapidly in a short time after inoculation in -OE plants. What is more, overexpression of enhanced the transcript levels of defense-related marker genes, such as , , , , and in -OE plants after inoculation. The results suggested that played an important role in regulating the expression of defense-related genes and the antioxidant enzymes, which resulted in resistance to infection.
E3泛素连接酶是泛素化过程中最重要的部分，参与植物免疫反应的各种过程。RBR E3连接酶是E3家族成员之一，但其在植物免疫中的功能仍鲜为人知。基于序列分析，NtRNF217是烟草中的一种RBR E3连接酶。为了评估其在烟草对（某种病原体，原文未明确写出）反应中的作用，在（云烟87，一个易感品种）上进行了过表达实验。结果表明，与野生型植物相比，过表达（该基因，原文未明确写出）的烟草显著降低了（某种病原体，原文未明确写出）的繁殖，并抑制了病害症状的发展。病原体接种后，（该基因，原文未明确写出）过表达植株中HO和O的积累显著高于野生型云烟87植株。在（该基因，原文未明确写出）过表达植株中接种（某种病原体，原文未明确写出）后短时间内，CAT和SOD的活性也迅速增加。此外，（该基因，原文未明确写出）的过表达增强了接种（某种病原体，原文未明确写出）后（该基因，原文未明确写出）过表达植株中防御相关标记基因如（多个基因，原文未明确写出）的转录水平。结果表明，（该基因，原文未明确写出）在调节防御相关基因的表达和抗氧化酶方面发挥了重要作用，从而导致对（某种病原体，原文未明确写出）感染具有抗性。
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