Transcriptomic Response of Resistant (PI613981-) and Susceptible ("Royal Gala") Genotypes of Apple to Blue Mold () Infection.

from Central Asia is a progenitor of the modern domesticated apple ( × ). Several accessions of are highly resistant to the postharvest pathogen . A previous study identified the qM-.1 QTL on LG3 for resistance to in the mapping population GMAL4593, developed using the resistant accession, -PI613981, and the susceptible cultivar "Royal Gala" (RG) (), as parents. The goal of the present study was to characterize the transcriptomic response of susceptible RG and resistant PI613981 apple fruit to wounding and inoculation with using RNA-Seq. Transcriptomic analyses 0-48 h post inoculation suggest a higher basal level of resistance and a more rapid and intense defense response to wounding and wounding plus inoculation with in -PI613981 than in RG. Functional analysis showed that ethylene-related genes and genes involved in "jasmonate" and "MYB-domain transcription factor family" were over-represented in the resistant genotype. It is suggested that the more rapid response in the resistant genotype (PI613981) plays a major role in the resistance response. At least twenty DEGs were mapped to the qM-3.1 QTL ( × v.1: 26,848,396-28,424,055) on LG3, and represent potential candidate genes responsible for the observed resistance QTL in -PI613981. RT-qPCR of several of these genes was used to validate the RNA-Seq data and to confirm their higher expression in MS0.