• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

人牙髓干细胞在生物活性骨磷灰石薄膜上的成骨分化

Osteogenic Differentiation of hDPSCs on Biogenic Bone Apatite Thin Films.

作者信息

Bianchi Michele, Pisciotta Alessandra, Bertoni Laura, Berni Matteo, Gambardella Alessandro, Visani Andrea, Russo Alessandro, de Pol Anto, Carnevale Gianluca

机构信息

Rizzoli Orthopaedic Institute, NanoBiotechnology Laboratory, Via di Barbiano 1/10, 40136 Bologna, Italy.

2Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Via del Pozzo 71, 41124 Modena, Italy.

出版信息

Stem Cells Int. 2017;2017:3579283. doi: 10.1155/2017/3579283. Epub 2017 Oct 22.

DOI:10.1155/2017/3579283
PMID:29201060
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5671751/
Abstract

A previous study reported the structural characterization of biogenic apatite (BAp) thin films realized by a pulsed electron deposition system by ablation of deproteinized bovine bone. Thin films annealed at 400°C exhibited composition and crystallinity degree very close to those of biogenic apatite; this affinity is crucial for obtaining faster osseointegration compared to conventional, thick hydroxyapatite (HA) coatings, for both orthopedics and dentistry. Here, we investigated the adhesion, proliferation, and osteogenic differentiation of human dental pulp stem cells (hDPCS) on as-deposited and heat-treated BAp and stoichiometric HA. First, we showed that heat-treated BAp films can significantly promote hDPSC adhesion and proliferation. Moreover, hDPSCs, while initially maintaining the typical fibroblast-like morphology and stemness surface markers, later started expressing osteogenic markers such as Runx-2 and OSX. Noteworthy, when cultured in an osteogenic medium on annealed BAp films, hDPSCs were also able to reach a more mature and terminal commitment, with respect to HA and as-deposited films. Our findings suggest that annealed BAp films not only preserve the typical biological properties of stemness of, hDPSCs but also improve their ability of osteogenic commitment.

摘要

先前的一项研究报道了通过脉冲电子沉积系统对脱蛋白牛骨进行烧蚀制备的生物成因磷灰石（BAp）薄膜的结构特征。在400°C退火的薄膜表现出与生物成因磷灰石非常接近的组成和结晶度；这种相似性对于在骨科和牙科领域中，与传统的厚羟基磷灰石（HA）涂层相比，获得更快的骨整合至关重要。在此，我们研究了人牙髓干细胞（hDPCS）在沉积态和热处理态的BAp以及化学计量比的HA上的粘附、增殖和成骨分化情况。首先，我们表明热处理的BAp薄膜能够显著促进hDPSC的粘附和增殖。此外，hDPSCs虽然最初保持典型的成纤维细胞样形态和干性表面标志物，但后来开始表达成骨标志物，如Runx-2和OSX。值得注意的是，当在成骨培养基中在退火的BAp薄膜上培养时，相对于HA和沉积态薄膜，hDPSCs也能够达到更成熟和终末的分化状态。我们的研究结果表明，退火的BAp薄膜不仅保留了hDPSCs典型的干性生物学特性，还提高了它们的成骨分化能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e01/5671751/193bf09e4e51/SCI2017-3579283.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e01/5671751/06a148e36e0a/SCI2017-3579283.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e01/5671751/4da8a3b7d1eb/SCI2017-3579283.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e01/5671751/3a34b222eaf2/SCI2017-3579283.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e01/5671751/b130f87456e6/SCI2017-3579283.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e01/5671751/193bf09e4e51/SCI2017-3579283.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e01/5671751/06a148e36e0a/SCI2017-3579283.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e01/5671751/4da8a3b7d1eb/SCI2017-3579283.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e01/5671751/3a34b222eaf2/SCI2017-3579283.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e01/5671751/b130f87456e6/SCI2017-3579283.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e01/5671751/193bf09e4e51/SCI2017-3579283.005.jpg

相似文献

1
Osteogenic Differentiation of hDPSCs on Biogenic Bone Apatite Thin Films.人牙髓干细胞在生物活性骨磷灰石薄膜上的成骨分化
Stem Cells Int. 2017;2017:3579283. doi: 10.1155/2017/3579283. Epub 2017 Oct 22.
2
Fabrication and characterization of biomimetic hydroxyapatite thin films for bone implants by direct ablation of a biogenic source.通过生物源的直接烧蚀制备用于骨植入物的仿生羟基磷灰石薄膜及其性能表征。
Mater Sci Eng C Mater Biol Appl. 2019 Jun;99:853-862. doi: 10.1016/j.msec.2019.02.033. Epub 2019 Feb 11.
3
Effects of a Novel Bioactive Glass Composition on Biological Properties of Human Dental Pulp Stem Cells.一种新型生物活性玻璃组合物对人牙髓干细胞生物学特性的影响。
Materials (Basel). 2020 Sep 12;13(18):4049. doi: 10.3390/ma13184049.
4
The effect of TRPM7 suppression on the proliferation, migration and osteogenic differentiation of human dental pulp stem cells.TRPM7 抑制对人牙髓干细胞增殖、迁移和成骨分化的影响。
Int Endod J. 2014 Jun;47(6):583-93. doi: 10.1111/iej.12193. Epub 2013 Oct 18.
5
[Osteogenic stimulation of human dental pulp stem cells with gelatin-hydroxyapatite-tricalcium phosphate scaffold].[明胶-羟基磷灰石-磷酸三钙支架对人牙髓干细胞的成骨刺激作用]
Shanghai Kou Qiang Yi Xue. 2020 Oct;29(5):492-498.
6
Polymeric vs hydroxyapatite-based scaffolds on dental pulp stem cell proliferation and differentiation.基于聚合物与羟基磷灰石的支架对牙髓干细胞增殖和分化的影响
World J Stem Cells. 2015 Nov 26;7(10):1215-21. doi: 10.4252/wjsc.v7.i10.1215.
7
Peroxiredoxin 6 Inhibits Osteogenic Differentiation and Bone Formation Through Human Dental Pulp Stem Cells and Induces Delayed Bone Development.过氧化物酶 6 通过人牙髓干细胞抑制成骨分化和骨形成，并诱导骨发育延迟。
Antioxid Redox Signal. 2019 Jun 10;30(17):1969-1982. doi: 10.1089/ars.2018.7530. Epub 2018 Jun 29.
8
Osteogenic Potential of Human Dental Pulp Stem Cells (hDPSCs) Growing on Poly L-Lactide-Co-Caprolactone and Hyaluronic Acid (HYAFF-11) Scaffolds.人牙髓干细胞（hDPSCs）在聚 L-丙交酯-共-己内酯和透明质酸（HYAFF-11）支架上的成骨潜力。
Int J Mol Sci. 2023 Nov 25;24(23):16747. doi: 10.3390/ijms242316747.
9
Characterization of a Stemness-Optimized Purification Method for Human Dental-Pulp Stem Cells: An Approach to Standardization.人牙髓干细胞干性优化纯化方法的鉴定：标准化方法。
Cells. 2022 Oct 12;11(20):3204. doi: 10.3390/cells11203204.
10
Characterization of Biological Properties of Dental Pulp Stem Cells Grown on an Electrospun Poly(l-lactide--caprolactone) Scaffold.在静电纺丝聚（L-丙交酯-己内酯）支架上培养的牙髓干细胞生物学特性的表征
Materials (Basel). 2022 Mar 3;15(5):1900. doi: 10.3390/ma15051900.

引用本文的文献

1
State of Art and Perspective of Calcium Phosphate-Based Coatings Coupled with Bioactive Compounds for Orthopedic Applications.用于骨科应用的磷酸钙基涂层与生物活性化合物结合的现状与展望
Nanomaterials (Basel). 2025 Aug 5;15(15):1199. doi: 10.3390/nano15151199.
2
Synthetic or Natural (Bio-Based) Hydroxyapatite? A Systematic Comparison between Biomimetic Nanostructured Coatings Produced by Ionized Jet Deposition.合成羟基磷灰石还是天然（生物基）羟基磷灰石？通过电离喷射沉积制备的仿生纳米结构涂层之间的系统比较。
Nanomaterials (Basel). 2024 Aug 9;14(16):1332. doi: 10.3390/nano14161332.
3
Ionized Jet Deposition of Calcium Phosphates-Based Nanocoatings: Tuning Coating Properties and Cell Behavior by Target Composition and Substrate Heating.

本文引用的文献

1
Understanding the role of nano-topography on the surface of a bone-implant.了解纳米形貌在骨植入物表面的作用。
Biomater Sci. 2013 Feb 3;1(2):135-151. doi: 10.1039/c2bm00032f. Epub 2012 Sep 26.
2
Calcium orthophosphate coatings, films and layers.正磷酸钙涂层、薄膜和层。
Prog Biomater. 2012 Sep 26;1(1):1. doi: 10.1186/2194-0517-1-1.
3
Ion-substituted calcium phosphate coatings deposited by plasma-assisted techniques: A review.等离子体辅助技术沉积的离子取代磷酸钙涂层：综述。
基于磷酸钙的纳米涂层的离子化喷射沉积：通过目标成分和基底加热调整涂层性能及细胞行为。
Nanomaterials (Basel). 2023 May 29;13(11):1758. doi: 10.3390/nano13111758.
4
PEDOT: PSS promotes neurogenic commitment of neural crest-derived stem cells.聚（3,4-乙撑二氧噻吩）：聚苯乙烯磺酸盐促进神经嵴衍生干细胞向神经源性方向分化。
Front Physiol. 2022 Aug 17;13:930804. doi: 10.3389/fphys.2022.930804. eCollection 2022.
5
Characterization of Dental Pulp Stem Cells Response to Bone Substitutes Biomaterials in Dentistry.牙科中牙髓干细胞对骨替代生物材料反应的表征
Polymers (Basel). 2022 May 30;14(11):2223. doi: 10.3390/polym14112223.
6
Innovative Nanomaterials for Biomedical Applications.用于生物医学应用的创新纳米材料。
Nanomaterials (Basel). 2022 May 5;12(9):1561. doi: 10.3390/nano12091561.
7
Evaluation of the In Vitro Biocompatibility of PEDOT:Nafion Coatings.聚（3,4-乙撑二氧噻吩）：全氟磺酸涂层的体外生物相容性评估
Nanomaterials (Basel). 2021 Aug 8;11(8):2022. doi: 10.3390/nano11082022.
8
Role of ascorbic acid in the regulation of epigenetic processes induced by Porphyromonas gingivalis in endothelial-committed oral stem cells.抗坏血酸在牙龈卟啉单胞菌诱导的内皮定向口腔干细胞表观遗传过程调节中的作用。
Histochem Cell Biol. 2021 Nov;156(5):423-436. doi: 10.1007/s00418-021-02014-8. Epub 2021 Aug 2.
9
Ionized jet deposition of antimicrobial and stem cell friendly silver-substituted tricalcium phosphate nanocoatings on titanium alloy.在钛合金上通过电离喷射沉积制备对抗菌和干细胞友好的银取代磷酸三钙纳米涂层。
Bioact Mater. 2021 Jan 5;6(8):2629-2642. doi: 10.1016/j.bioactmat.2020.12.019. eCollection 2021 Aug.
10
Effects of the Sintering Process on Nacre-Derived Hydroxyapatite Scaffolds for Bone Engineering.烧结工艺对用于骨工程的珍珠层衍生羟基磷灰石支架的影响。
Molecules. 2020 Jul 8;25(14):3129. doi: 10.3390/molecules25143129.
Mater Sci Eng C Mater Biol Appl. 2017 May 1;74:219-229. doi: 10.1016/j.msec.2016.12.018. Epub 2016 Dec 8.
4
Human dental pulp stem cells expressing STRO-1, c-kit and CD34 markers in peripheral nerve regeneration.人牙髓干细胞在周围神经再生中表达 STRO-1、c-kit 和 CD34 标志物。
J Tissue Eng Regen Med. 2018 Feb;12(2):e774-e785. doi: 10.1002/term.2378. Epub 2017 Mar 2.
5
Surface morphology, tribological properties and in vitro biocompatibility of nanostructured zirconia thin films.纳米结构氧化锆薄膜的表面形态、摩擦学性能及体外生物相容性
J Mater Sci Mater Med. 2016 May;27(5):96. doi: 10.1007/s10856-016-5707-4. Epub 2016 Mar 22.
6
Tribological characterization of zirconia coatings deposited on Ti6Al4V components for orthopedic applications.用于骨科应用的Ti6Al4V部件上沉积的氧化锆涂层的摩擦学特性。
Mater Sci Eng C Mater Biol Appl. 2016 May;62:643-55. doi: 10.1016/j.msec.2016.02.014.
7
Neural crest: The fourth germ layer.神经嵴：第四胚层。
J Oral Maxillofac Pathol. 2015 May-Aug;19(2):221-9. doi: 10.4103/0973-029X.164536.
8
Stem cells isolated from human dental pulp and amniotic fluid improve skeletal muscle histopathology in mdx/SCID mice.从人牙髓和羊水中分离出的干细胞可改善mdx/SCID小鼠的骨骼肌组织病理学。
Stem Cell Res Ther. 2015 Aug 28;6(1):156. doi: 10.1186/s13287-015-0141-y.
9
Human dental pulp stem cells (hDPSCs): isolation, enrichment and comparative differentiation of two sub-populations.人牙髓干细胞（hDPSCs）：两个亚群的分离、富集及比较分化
BMC Dev Biol. 2015 Mar 14;15:14. doi: 10.1186/s12861-015-0065-x.
10
Hydroxyapatite coating does not improve uncemented stem survival after total hip arthroplasty!羟基磷灰石涂层并不能提高全髋关节置换术后非骨水泥柄的存活率！
Acta Orthop. 2015 Feb;86(1):18-25. doi: 10.3109/17453674.2014.957088. Epub 2014 Sep 1.