Graziani Gabriela, Barbaro Katia, Fadeeva Inna V, Ghezzi Daniele, Fosca Marco, Sassoni Enrico, Vadalà Gianluca, Cappelletti Martina, Valle Francesco, Baldini Nicola, Rau Julietta V
IRCSS Istituto Ortopedico Rizzoli, Laboratory of NanoBiotechnology, via di Barbiano 1/10 40136, Bologna, Italy.
Istituto Zooprofilattico Sperimentale Lazio e Toscana "M. Aleandri", Via Appia Nuova 1411-00178, Rome, Italy.
Bioact Mater. 2021 Jan 5;6(8):2629-2642. doi: 10.1016/j.bioactmat.2020.12.019. eCollection 2021 Aug.
Orthopedic infections pose severe societal and economic burden and interfere with the capability of the implanted devices to integrate in the host bone, thus significantly increasing implants failure rate. To address infection and promote integration, here nanostructured antibacterial and bioactive thin films are proposed, obtained, for the first time, by Ionized Jet Deposition (IJD) of silver-substituted tricalcium phosphate (Ag-TCP) targets on titanium. Coatings morphology, composition and mechanical properties are characterized and proof-of-concept of biocompatibility is shown. Antimicrobial efficacy is investigated against four Gram positive and Gram negative bacterial strains and against fungus, by investigating the modifications in planktonic bacterial growth in the absence and presence of silver. Then, for all bacterial strains, the capability of the film to inhibit bacterial adhesion is also tested. Results indicate that IJD permits a fine control over films composition and morphology and deposition of films with suitable mechanical properties. Biological studies show a good efficacy against and against fungus , with evidences of efficacy against planktonic growth and significant reduction of bacterial cell adhesion. No cytotoxic effects are evidenced for equine adipose tissue derived mesenchymal stem cells (ADMSCs), as no reductions are caused to cells viability and no interference is assessed in cells differentiation towards osteogenic lineage, in the presence of silver. Instead, thanks to nanostructuration and biomimetic composition, tricalcium phosphate (TCP) coatings favor cells viability, also when silver-substituted. These findings show that silver-substituted nanostructured coatings are promising for orthopedic implant applications.
骨科感染带来了严重的社会和经济负担，并干扰植入装置与宿主骨整合的能力，从而显著提高植入物的失败率。为了解决感染问题并促进整合，本文提出了一种纳米结构的抗菌和生物活性薄膜，该薄膜首次通过在钛上离子化喷射沉积（IJD）银取代的磷酸三钙（Ag-TCP）靶材获得。对涂层的形态、成分和力学性能进行了表征，并展示了生物相容性的概念验证。通过研究在有无银存在的情况下浮游细菌生长的变化，研究了对四种革兰氏阳性和革兰氏阴性细菌菌株以及真菌的抗菌效果。然后，针对所有细菌菌株，还测试了薄膜抑制细菌粘附的能力。结果表明，IJD能够精细控制薄膜的成分和形态，并沉积具有合适力学性能的薄膜。生物学研究表明，该薄膜对[具体细菌名称]和真菌具有良好的抗菌效果，对浮游生长有明显的抗菌效果，并显著减少细菌细胞粘附。对于马脂肪组织来源的间充质干细胞（ADMSCs），未发现细胞毒性作用，因为在有银存在的情况下，细胞活力没有降低，也没有评估对细胞向成骨谱系分化的干扰。相反，由于纳米结构和仿生成分，磷酸三钙（TCP）涂层即使在银取代的情况下也有利于细胞活力。这些发现表明，银取代的纳米结构涂层在骨科植入物应用中具有广阔前景。
