Chahardoli Azam, Karimi Naser, Fattahi Ali
Laboratory of plant physiology, Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran.
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Iran J Pharm Res. 2017 Summer;16(3):1167-1175.
The biogenic synthesis of metal nanomaterial offers an environmentally benign alternative to the traditional chemical synthesis routes. In the present study, the green synthesis of silver nanoparticles (AgNPs) from aqueous solution of silver nitrate (AgNO) by using L. seed powder extract (NSPE) has been reported. AgNPs were characterized by UV-vis absorption spectroscopy with an intense surface plasmon resonance band at 435 nm which reveals the formation of nanoparticles. Fourier transmission infrared spectroscopy (FTIR) showed that nanoparticles were capped with plant compounds. Transmission electron microscopy (TEM) showed silver nanoparticles, with a size of 2-15 nm, were spherical. The X-ray diffraction spectrum (XRD) pattern clearly indicates that AgNPs formed in the present synthesis were crystalline in nature. Stabilized films of exudate synthesized AgNPs were effective anti-bacterial agents. In addition, these biologically synthesized nanoparticles were also proved to exhibit excellent cytotoxic effect on a human breast cancer cell line (MCF-7) and a human colorectal adenocarcinoma cell line (HT-29). The results confirmed that the NSPE is a very good ecofriendly and nontoxic source for the synthesis of AgNPs as compared to the conventional chemical/physical methods. Therefore, seed provides future opportunities in nanomedicine by tagging nanoparticles with secondary metabolites.
金属纳米材料的生物合成提供了一种环境友好的传统化学合成路线替代方案。在本研究中，已报道了利用罗勒种子粉末提取物（NSPE）从硝酸银（AgNO）水溶液中绿色合成银纳米颗粒（AgNPs）。通过紫外可见吸收光谱对AgNPs进行表征，在435nm处有一个强烈的表面等离子体共振带，这表明纳米颗粒的形成。傅里叶透射红外光谱（FTIR）表明纳米颗粒被植物化合物包覆。透射电子显微镜（TEM）显示银纳米颗粒呈球形，尺寸为2 - 15nm。X射线衍射光谱（XRD）图谱清楚地表明本合成中形成的AgNPs本质上是结晶的。渗出液合成的AgNPs的稳定膜是有效的抗菌剂。此外，这些生物合成的纳米颗粒还被证明对人乳腺癌细胞系（MCF - 7）和人大肠腺癌细胞系（HT - 29）表现出优异的细胞毒性作用。结果证实，与传统化学/物理方法相比，NSPE是合成AgNPs的一种非常好的生态友好且无毒的来源。因此，罗勒种子通过用次生代谢物标记纳米颗粒为纳米医学提供了未来的机会。
