Guo Jing-Jing, Xu Hong-Shun, Wu Gang, Ma Zhen-Yu
Department of General Surgery, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
Department of General Surgery, Renhe Hospital, Shanghai 200043, P.R. China.
Exp Ther Med. 2017 Nov;14(5):4693-4696. doi: 10.3892/etm.2017.5137. Epub 2017 Sep 19.
Thyroid cancer typically has a good prognosis; however, the risks of recurrence and chemoresistance associated with thyroid cancer remain a cause for concern. Papillary thyroid carcinoma (PTC) comprises 80% of all cases of thyroid carcinoma. A previous study reported that cluster of differentiation (CD) 44/CD24 PTC cells may contribute to PTC recurrence and chemoresistance; however, the underlying molecular mechanisms remain elusive. In the present study, CD44/CD24 cells were isolated from the TPC-1 PTC cell line and biological function assays revealed that CD44/CD24 cells were significantly more proliferative and chemoresistant compared with CD44/CD24 cells. Furthermore, the expression level of p63 was demonstrated to be negatively correlated with the expression of CD44 in PTC cells. The role of p63 in CD44/CD24 cell proliferation and chemoresistance was investigated and, the ectopic expression of p63 was observed to significantly inhibit CD44/CD24 cell proliferation and chemoresistance and . In conclusion, the present study indicated that CD44/CD24 cells contribute to PTC proliferation and chemoresistance and that the suppression of p63 in CD44/CD24 cells contributes to these effects.
甲状腺癌通常预后良好；然而，与甲状腺癌相关的复发和化疗耐药风险仍然令人担忧。甲状腺乳头状癌（PTC）占所有甲状腺癌病例的80%。先前的一项研究报告称，分化簇（CD）44/CD24 PTC细胞可能导致PTC复发和化疗耐药；然而，其潜在的分子机制仍然不清楚。在本研究中，从TPC-1 PTC细胞系中分离出CD44/CD24细胞，生物学功能分析显示，与CD44/CD24细胞相比，CD44/CD24细胞具有显著更高的增殖能力和化疗耐药性。此外，p63的表达水平被证明与PTC细胞中CD44的表达呈负相关。研究了p63在CD44/CD24细胞增殖和化疗耐药中的作用，观察到p63的异位表达显著抑制CD44/CD24细胞的增殖和化疗耐药性。总之，本研究表明，CD44/CD24细胞促进PTC的增殖和化疗耐药性，并且CD44/CD24细胞中p63的抑制促成了这些效应。
