Fu Gen-Feng, Tian She-Min, Cha Xin-Jian, Huang Hong-Jun, Lou Ji-He, Wei Ying, Xia Cheng-De, Li Yong-Lin, Niu Xi-Hua
Department of Burn and Plastic Surgery, Taihe Hospital, Changsha, Hunan 410005, P.R. China.
Department of Burns, The First People's Hospital of Zhengzhou City, Zhengzhou, Henan 450004, P.R. China.
Exp Ther Med. 2017 Nov;14(5):4825-4830. doi: 10.3892/etm.2017.5146. Epub 2017 Sep 20.
Using a rat comb thermal damage model, we investigated the effects of topically administered recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) on peroxisome proliferator-activated receptor PPARβ expression. We created bilateral comb scald models on the backs of fifty Sprague-Dawley rats. The left sides of the backs served as the experimental group and the right sides served as the control group. The experimental group received topically applied rhGM-CSF hydrogel and the control group did not. The survival situations of the stasis zones were compared between the experimental and control groups on the 1st, 3rd, 7th, 14th and 21st post-burn days. Tissues from the surviving stasis zones of both groups were collected at different time-points. Reverse transcriptase-polymerase chain reaction (RT-PCR) and western blotting were used to detect the PPARβ mRNA and protein expression levels. Immunohistochemical methods were applied to detect the localization of PPARβ protein expression. The results showed that, first, the tissue viability numbers for the stasis zones of the experimental group were significantly increased compared with those of the control group. Second, RT-PCR revealed that the PPARβ mRNA expression first increased and then gradually declined in both groups. At all time-points, the expression level in the experimental group was increased compared with that in the control group and the highest expression levels were observed in both groups on the 3rd post-burn day. Third, western blot analysis revealed that the PPARβ protein expression in both groups increased after thermal damage and then gradually decreased. PPARβ protein expression in the experimental group was greater than that in the control group, and the highest expression quantities in both groups were observed on the 3rd post-burn day. In conclusion, rhGM-CSF hydrogel effectively promotes the expression of PPARβ, and the hydrogel had a specific protective effect for the stasis zone.
利用大鼠梳齿热损伤模型，我们研究了局部应用重组人粒细胞巨噬细胞集落刺激因子（rhGM-CSF）对过氧化物酶体增殖物激活受体PPARβ表达的影响。我们在50只Sprague-Dawley大鼠背部建立双侧梳齿烫伤模型。背部左侧作为实验组，右侧作为对照组。实验组局部应用rhGM-CSF水凝胶，对照组未应用。比较实验组和对照组在烧伤后第1、3、7、14和21天淤滞区的存活情况。在不同时间点收集两组存活淤滞区的组织。采用逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹法检测PPARβ mRNA和蛋白表达水平。应用免疫组织化学方法检测PPARβ蛋白表达的定位。结果显示，第一，实验组淤滞区的组织存活数量与对照组相比显著增加。第二，RT-PCR显示两组中PPARβ mRNA表达均先升高后逐渐下降。在所有时间点，实验组的表达水平均高于对照组，且两组在烧伤后第3天观察到最高表达水平。第三，蛋白质免疫印迹分析显示，热损伤后两组中PPARβ蛋白表达均增加，然后逐渐降低。实验组中PPARβ蛋白表达高于对照组，且两组在烧伤后第3天观察到最高表达量。总之，rhGM-CSF水凝胶有效促进PPARβ的表达，且该水凝胶对淤滞区具有特异性保护作用。
