Department of Plastic, Reconstruction and Aesthetic Surgery, İnönü Univesity, Medical Faculty, Malatya, Turkey.
Department of Histology and Embryology, İnönü Univesity, Medical Faculty, Malatya, Turkey.
Burns. 2022 Dec;48(8):1850-1862. doi: 10.1016/j.burns.2022.01.005. Epub 2022 Jan 7.
Among the zones of coagulation, hyperemia and stasis that occur in the burned area, the most intense metabolic process and the highest sensitivity to recovery with treatment is the zone of stasis.This metabolic process is related to how well the tissues in the zone of stasis can cope with oxidative stress. If the tissues in the zone of stasis are saved, the burn area will potentially heal faster and with less scar. In this study, we examined the effects of taurine amino acids and apocynin molecules on saving the tissues in the burn zone of stasis.
The study was conducted with 48 rats. The burn zone of stasis was created according to the pattern previously described in the literature as comb burn model. In the 21-day study, biopsies were taken for histological examination on the 3rd, 7th and 21st days of the study. In addition, macroscopic photographic analysis was performed. Biopsies were taken for biochemical analysis on the 21st day. Histologically, inflammation, reepithelialization, and collagenization were evaluated, and the CD34 immunoreactivity was analysed. Biochemically, CAT, SOD, tGSH, TAS, TOS, MDA and PPC values were determined.
In the histological examination, on the 3rd, 7th and 21st days, inflammation was found to be reduced in the groups given taurine and apocynin on the 3rd day. On the 7th day, better reepithelialization and collagenization were observed in the group given taurine. Significant reepithelialization, collagenization and hair follicle development were observed in the groups given taurine and apocynin on the 21st day. In the biochemical analysis, the effects of apocynin on antioxidant enzymes were determined to be prominent. While we found no significant difference on the 3rd and 7th days in the photographic analysis, taurine and apocynin were observed to act synergistically on the 21st day and significantly reduced the burned areas.
On the 3rd day, taurine and apocynin prevented inflammation, the effects of taurine in the zone of stasis in the early period (7th day) are more pronounced, the effect of apocynin on antioxidant enzymes is more pronounced. In the late period (21st day), taurine and apocynin were found to be more effective in saving the zone of stasis by creating a synergistic effect.
在烧伤区域的凝血、充血和淤血区中，代谢过程最剧烈且对治疗恢复最敏感的是淤血区。这种代谢过程与淤血区组织对氧化应激的适应能力有关。如果淤血区的组织得以保存，烧伤区域的愈合速度将会更快，且疤痕更少。在这项研究中，我们研究了牛磺酸氨基酸和阿朴肉桂醇分子对保存烧伤淤血区组织的影响。
本研究使用了 48 只大鼠。根据文献中先前描述的 Comb 烧伤模型，创建了淤血区烧伤模型。在 21 天的研究中，在研究的第 3、7 和 21 天分别进行组织学检查活检。此外，还进行了宏观摄影分析。在第 21 天进行了生化分析。组织学上评估了炎症、再上皮化和胶原化，并分析了 CD34 免疫反应性。生化分析测定 CAT、SOD、tGSH、TAS、TOS、MDA 和 PPC 值。
在组织学检查中，在第 3、7 和 21 天，在第 3 天给予牛磺酸和阿朴肉桂醇的组中，炎症减少。在第 7 天，给予牛磺酸的组中再上皮化和胶原化更好。在第 21 天，给予牛磺酸和阿朴肉桂醇的组中观察到明显的再上皮化、胶原化和毛囊发育。在生化分析中，阿朴肉桂醇对抗氧化酶的影响明显。虽然在第 3 天和第 7 天的摄影分析中没有发现显著差异，但在第 21 天，牛磺酸和阿朴肉桂醇表现出协同作用，显著减少了烧伤面积。
在第 3 天，牛磺酸和阿朴肉桂醇预防了炎症，牛磺酸在早期（第 7 天）淤血区的作用更明显，阿朴肉桂醇对抗氧化酶的作用更明显。在后期（第 21 天），牛磺酸和阿朴肉桂醇通过产生协同作用，在保存淤血区方面更有效。
