Wei Chengjun, Zhang Hongxia, Wei Chenghong, Mao Youyan
Department of Laboratory Medicine, Weifang People's Hospital, Weifang, Shandong 261041, P.R. China.
Department of Laboratory Medicine, Weifang Traditional Chinese Hospital, Weifang, Shandong 261041, P.R. China.
Exp Ther Med. 2017 Nov;14(5):5027-5031. doi: 10.3892/etm.2017.5155. Epub 2017 Sep 21.
We investigated the expression of miR-146a in peripheral blood mononuclear cell (PBMC) of patients with ankylosing spondylitis (AS) and its correlation with inflammatory factors to explore the clinical significance. In total 45 patients with AS were selected at the Weifang People's Hospital from June, 2014 to January, 2016. At the same time, 30 healthy volunteers were also selected to serve as control group. Expression level of miR-146a in PBMC cells of patients in each group was detected by quantitative real-time-polymerase chain reaction (qRT-PCR). Levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and IL-6 in serum and the supernatant of culture medium of PBMC derived from each group were detected by enzyme-linked immunosorbent assay (ELISA). Correlations between expression level of miR-146a and serum inflammatory factors, and clinical indicators were analyzed. Clinical indicators included bath ankylosing spondylitis disease activity index (BASDAI), C-reactive protein (CRP), erythrocyte sedimentation rate (ESR) and duration of morning stiffness. Expression level of miR-146a in PBMC of AS patients was significantly higher than that of healthy control (P<0.01); levels of TNF-α, IL-1β and IL-6 in serum and the supernatant of culture medium of PBMC derived from AS patients were significant compared to those of control group (P<0.01); expression of miR-146a in PBMC of patients with AS was positively correlated with the levels of TNF-α, IL-1β and IL-6 in serum (r=0.632, P<0.01; r=0.574, P<0.01; r=0.483, P<0.01). In addition, expression level of miR-146a in PBMC of patients with AS was positively correlated with BASDAI, ESR, CRP and duration of morning stiffness (r=0.551, P<0.01; r=0.738, P<0.01; r=0.685, P<0.01; r=0.497, P<0.01). Expression level of miR-146a in PBMC of AS patients was significantly increased and the expression level was positively correlated with the levels of TNF-α, IL-1β and IL-6 in serum (P<0.05). In addition, expression level of miR-146a in PBMC of AS patients was also positively correlated with BASDAI, ESR, CRP and duration of morning stiffness. Those results suggest that miR-146a may be involved in the pathogenesis of AS, and the expression level of miR-146a in PBMC cells may be helpful for diagnosis of AS and judgment of disease activity.
我们研究了强直性脊柱炎（AS）患者外周血单个核细胞（PBMC）中miR-146a的表达及其与炎症因子的相关性，以探讨其临床意义。2014年6月至2016年1月期间，在潍坊市人民医院共选取了45例AS患者。同时，还选取了30名健康志愿者作为对照组。采用定量实时聚合酶链反应（qRT-PCR）检测每组患者PBMC细胞中miR-146a的表达水平。采用酶联免疫吸附测定（ELISA）检测每组患者血清及PBMC培养基上清中肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和IL-6的水平。分析miR-146a表达水平与血清炎症因子及临床指标之间的相关性。临床指标包括巴氏强直性脊柱炎疾病活动指数（BASDAI）、C反应蛋白（CRP）、红细胞沉降率（ESR）和晨僵持续时间。AS患者PBMC中miR-146a的表达水平显著高于健康对照组（P<0.01）；与对照组相比，AS患者血清及PBMC培养基上清中TNF-α、IL-1β和IL-6的水平显著升高（P<0.01）；AS患者PBMC中miR-146a的表达与血清中TNF-α、IL-1β和IL-6的水平呈正相关（r=0.632，P<0.01；r=0.574，P<0.01；r=0.483，P<0.01）。此外，AS患者PBMC中miR-146a的表达水平与BASDAI、ESR、CRP和晨僵持续时间呈正相关（r=0.551，P<0.01；r=0.738，P<0.01；r=0.685，P<0.01；r=0.497，P<0.01）。AS患者PBMC中miR-146a的表达水平显著升高，且该表达水平与血清中TNF-α、IL-1β和IL-6的水平呈正相关（P<0.05）。此外，AS患者PBMC中miR-146a的表达水平还与BASDAI、ESR、CRP和晨僵持续时间呈正相关。这些结果表明，miR-146a可能参与了AS的发病机制，PBMC细胞中miR-146a的表达水平可能有助于AS的诊断和疾病活动度的判断。
