Li Jiangbi, Xie Xiaoping, Liu Weibing, Gu Feng, Zhang Ke, Su Zilong, Wen Qiangqiang, Sui Zhenjiang, Zhou Pengcheng, Yu Tiecheng
Department of Orthopedics, The First Hospital of Jilin University, Changchun, China.
Department of Orthopedics, The First People's Hospital of Yunnan Province, Kunming, China.
Front Med (Lausanne). 2021 Aug 10;8:701789. doi: 10.3389/fmed.2021.701789. eCollection 2021.
Abnormal expression levels of microRNAs (miRNAs) were observed in ankylosing spondylitis (AS) in recent articles, suggesting that miRNAs may be used as biomarkers for AS diagnoses. In this paper, we conducted a meta-analysis to identify the overall diagnostic accuracy of miRNA biomarkers in AS patients. An extensive search was undertaken in PubMed, Embase, Cochrane databases, and Wan Fang database up to 30 December 2020 using the following key words: ("microRNAs" or "microRNA" or "miRNA" or "miR" or "RNA, Micro" or "Primary MicroRNA") and ("Spondylitis Ankylosing" or "Spondyloarthritis Ankylopoietica" or "Ankylosing Spondylarthritis" or "Ankylosing Spondylarthritides" or "Spondylarthritides Ankylosing" or "Ankylosing Spondylitis") and ("blood" or "serum" or "plasma"). Statistical evaluation of dysregulated miRNAs using the sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and the area under the curve (AUC). Twenty-nine articles reporting on the miRNAs of AS were included. A total of 42 miRNAs were observed to be up-regulated and 45 miRNAs were down-regulated in the AS cases compared with the controls. Besides, 29 studies from nine articles were included in our meta-analysis. The pooled sensitivity, specificity, PLR, NLR, DOR, and AUC were 0. 76 (95% CI, 0.70-0.81), 0.80 (95% CI, 0.74-0.85), 3.75 (95% CI, 2.82-5.01), 0.30 (95% CI, 0.24-0.39), 12.32 (95% CI, 7.65-19.83), 0.85 (95% CI, 0.81-0.88), respectively, suggesting a good diagnostic accuracy of miRNAs for AS. Circulating miRNAs are deregulated in AS patients. miRNAs may be used as a relatively non-invasive biomarkers for the detection of AS.
近期文章显示，强直性脊柱炎（AS）患者体内的微小RNA（miRNA）表达水平异常，这表明miRNA有望作为AS诊断的生物标志物。本文通过荟萃分析来确定miRNA生物标志物对AS患者的总体诊断准确性。截至2020年12月30日，我们在PubMed、Embase、Cochrane数据库及万方数据库中进行了全面检索，检索关键词如下：（“微小RNA”或“微小核糖核酸”或“miRNA”或“miR”或“RNA，微小”或“初级微小RNA”）以及（“脊柱炎，强直性”或“强直性脊柱炎”或“脊柱关节炎，强直性”或“强直性脊柱炎”或“强直性脊椎炎”或“脊椎炎，强直性”或“强直性脊柱炎”）以及（“血液”或“血清”或“血浆”）。使用敏感性、特异性、阳性似然比（PLR）、阴性似然比（NLR）、诊断比值比（DOR）和曲线下面积（AUC）对失调的miRNA进行统计学评估。纳入了29篇关于AS患者miRNA的文章。与对照组相比，共观察到42种miRNA在AS病例中上调，45种miRNA下调。此外，我们的荟萃分析纳入了9篇文章中的29项研究。汇总的敏感性、特异性、PLR、NLR、DOR和AUC分别为0.76（95%CI，0.70 - 0.81）、0.80（95%CI，0.74 - 0.85）、3.75（95%CI，2.82 - 5.01）、0.30（95%CI，0.24 - 0.39）、12.32（95%CI，7.65 - 19.83）、0.85（95%CI，0.81 - 0.88），这表明miRNA对AS具有良好的诊断准确性。AS患者体内循环miRNA失调。miRNA可作为检测AS的相对非侵入性生物标志物。
