O'Rourke A M, Rider C C
Department of Biochemistry, Royal Holloway and Bedford New College, University of London, Egham, Surrey, U.K.
Biochim Biophys Acta. 1989 Mar 6;1010(3):342-5. doi: 10.1016/0167-4889(89)90059-1.
The utilisation of glucose, glutamine, acetoacetate and D-3-hydroxybutyrate were investigated over 72 h of incubation of rat splenic lymphocytes, with and without concanavalin A. Lymphocytes consumed both ketone bodies; acetoacetate was consumed preferentially. The ketone bodies reduced glucose consumption by 30-50%, but had little effect on lactate production. Glutamine uptake was concentration dependent up to 4 mM, and consumption was increased in the presence of concanavalin. Glutamine stimulated glucose consumption and lactate production in both resting and activated cells. Complete oxidation contributed 65% of glucose-derived ATP, but less than 40% of glutamine-derived ATP. Glutamine metabolism makes only a minor contribution to lymphocyte ATP generation.
在有或没有伴刀豆球蛋白A的情况下，对大鼠脾淋巴细胞进行72小时培养，研究了葡萄糖、谷氨酰胺、乙酰乙酸和D-3-羟基丁酸的利用情况。淋巴细胞消耗两种酮体；优先消耗乙酰乙酸。酮体使葡萄糖消耗减少30%-50%，但对乳酸生成影响不大。谷氨酰胺摄取在浓度高达4 mM时呈浓度依赖性，在伴刀豆球蛋白存在的情况下消耗量增加。谷氨酰胺刺激静息细胞和活化细胞中的葡萄糖消耗和乳酸生成。完全氧化对葡萄糖衍生的ATP贡献65%，但对谷氨酰胺衍生的ATP贡献不到40%。谷氨酰胺代谢对淋巴细胞ATP生成的贡献很小。
