Institute of Physiological Chemistry, University Hospital Essen, University Duisburg-Essen, Essen, Germany.
Biomed Res Int. 2017;2017:1072969. doi: 10.1155/2017/1072969. Epub 2017 Oct 19.
Intestinal ischemia is often caused by a malperfusion of the upper mesenteric artery. Since the intestinal mucosa is one of the most rapidly proliferating organs in human body, this tissue can partly regenerate itself after the onset of ischemia and reperfusion (I/R). Therefore, we investigated whether glycine, sodium pyruvate, and resveratrol can either support or potentially harm regeneration when applied therapeutically after reperfusion injury.
I/R of the small intestine was initiated by occluding and reopening the upper mesenteric artery in rats. After 60 min of ischemia and 300 min of reperfusion, glycine, sodium pyruvate, or resveratrol was administered intravenously. Small intestine regeneration was analyzed regarding tissue damage, activity of saccharase, and Ki-67 positive cells. Additionally, systemic parameters and metabolic ones were obtained at selected periods.
Resveratrol failed in improving the outcome after I/R, while glycine showed a partial beneficial effect. Sodium pyruvate ameliorated metabolic acidosis, diminished histopathologic tissue injury, and increased cell proliferation in the small intestine.
While glycine could improve in part regeneration but not proliferation, sodium pyruvate seems to be a possible therapeutic agent to facilitate proliferation and to support mucosal regeneration after I/R injury to the small intestine.
肠缺血通常是由肠系膜上动脉的灌注不良引起的。由于肠黏膜是人体中增殖最快的器官之一，因此在发生缺血再灌注（I/R）后，该组织可以部分自我再生。因此，我们研究了甘氨酸、丙酮酸钠和白藜芦醇在再灌注损伤后应用于治疗时，是否可以支持或潜在地损害再生。
通过夹闭和重新开放大鼠肠系膜上动脉来启动小肠的 I/R。在缺血 60 分钟和再灌注 300 分钟后，静脉给予甘氨酸、丙酮酸钠或白藜芦醇。分析组织损伤、蔗糖酶活性和 Ki-67 阳性细胞，以评估小肠再生。此外，在选定的时间段内获得全身和代谢参数。
白藜芦醇未能改善 I/R 后的结果，而甘氨酸显示出部分有益的效果。丙酮酸钠改善了代谢性酸中毒，减轻了组织损伤，增加了小肠的细胞增殖。
虽然甘氨酸部分改善了再生但没有促进增殖，而丙酮酸钠似乎是一种可能的治疗药物，可以促进增殖，并在 I/R 损伤后支持小肠黏膜的再生。
