Heenan M P, Nacey J N, Delahunt B, Ferguson A F, Dickson S J
Department of Scientific and Industrial Research, Wellington School of Medicine, New Zealand.
Br J Urol. 1989 Jan;63(1):72-5. doi: 10.1111/j.1464-410x.1989.tb05127.x.
Levels of volatile N-nitrosamines were measured in 10 brands of latex and 2 brands of silicone catheters using high performance liquid chromatography. The cytotoxicity of catheters from identical batches was determined by measuring the inhibitory effect of catheter extracts on the uptake of 3H-labelled thymidine into L-929 fibroblasts in culture (IC50). The most frequently encountered nitrosamines were N-nitrosodi-n-butylamine and N-nitrosodiethylamine. Total N-nitrosamine levels in excess of 100 ng/g were found in 6 of the 16 catheters tested. When compared with the cytotoxicity of the catheters a significant correlation was found, with increasing nitrosamine content being associated with greater cytotoxicity. In view of the reported toxic and carcinogenic effects of these compounds it is suggested that the nitrosamine content of catheters be routinely monitored and safe regulatory limits be imposed.
使用高效液相色谱法测量了10个品牌的乳胶导管和2个品牌的硅胶导管中挥发性N-亚硝胺的含量。通过测量导管提取物对3H标记的胸腺嘧啶核苷摄取到培养的L-929成纤维细胞中的抑制作用（IC50），来确定相同批次导管的细胞毒性。最常检测到的亚硝胺是N-亚硝基二正丁胺和N-亚硝基二乙胺。在测试的16根导管中，有6根的总N-亚硝胺含量超过100 ng/g。将导管的细胞毒性与之相比时，发现存在显著相关性，亚硝胺含量增加与更大的细胞毒性相关。鉴于这些化合物已报道的毒性和致癌作用，建议常规监测导管的亚硝胺含量并设定安全监管限值。
