Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
School of Life Sciences, Shandong University of Technology, Zibo, 255000, Shandong, China.
J Ind Microbiol Biotechnol. 2018 Jan;45(1):61-69. doi: 10.1007/s10295-017-1992-2. Epub 2017 Dec 4.
Renewable energy, including biofuels such as ethanol and butanol from syngas bioconversed by Clostridium carboxidivorans P7, has been drawing extensive attention due to the fossil energy depletion and global eco-environmental issues. Effects of zinc on the growth and metabolites of C. carboxidivorans P7 were investigated with model syngas as the carbon source. The cell concentration was doubled, the ethanol content increased 3.02-fold and the butanol content increased 7.60-fold, the hexanol content increased 44.00-fold in the medium with 280 μM Zn, when comparing with those in the control medium [Zn, (7 μM)]. Studies of the genes expression involved in the carbon fixation as well as acid and alcohol production in the medium with 280 μM Zn indicated that fdhII was up-regulated on the second day, acs A, fdhII, bdh35 and bdh50 were up-regulated on the third day and bdh35, acsB, fdhI, fdhIII, fdhIV, buk, bdh10, bdh35, bdh40 and bdh50 were up-regulated on the fourth day. The results indicated that the increased Zn content increased the alcohol production through increase in the gene expression of the carbon fixation and alcohol dehydrogenase.
由于化石能源枯竭和全球生态环境问题，包括由产电菌 Clostridium carboxidivorans P7 生物转化合成气生产的生物燃料乙醇和丁醇在内的可再生能源引起了广泛关注。本研究以模拟合成气为碳源，考察了锌对产电菌 C. carboxidivorans P7 生长和代谢产物的影响。结果表明，与对照培养基[Zn，（7 μM）]相比，培养基中添加 280 μM Zn 时细胞浓度增加了 1 倍，乙醇含量增加了 3.02 倍，丁醇含量增加了 7.60 倍，己醇含量增加了 44.00 倍。在添加 280 μM Zn 的培养基中研究与碳固定以及酸和醇生产相关的基因表达表明，第二天 fdhII 上调，第三天 acsA、fdhII、bdh35 和 bdh50 上调，第四天 bdh35、acsB、fdhI、fdhIII、fdhIV、buk、bdh10、bdh35、bdh40 和 bdh50 上调。结果表明，增加 Zn 含量通过增加碳固定和醇脱氢酶的基因表达来提高酒精产量。
