Machado Ricardo, Garcia Lucas da Fonseca Roberti, da Silva Neto Ulisses Xavier, Cruz Filho Antônio de Miranda da, Silva Ricardo Gariba, Vansan Luiz Pascoal
Department of Restorative Dentistry (Endodontics), School of Dentistry, University of São Paulo, Avenida do Café, s/n, Campus da USP Ribeirão, Ribeirão Preto, São Paulo CEP: 14040-904, Brazil.
Department of Dentistry (Endodontics Division), Federal University of Santa Catarina, Campus Reitor João David Ferreira Lima, s/n, Trindade, Florianópolis, Santa Catarina CEP: 88040-900, Brazil.
Microsc Res Tech. 2018 Mar;81(3):275-282. doi: 10.1002/jemt.22976. Epub 2017 Dec 5.
The purpose of this study was to compare the efficacy of different chelating solutions (17% EDTA and 10% citric acid) on the smear layer removal, and their effect on tubular dentin sealer penetration. Sixty root canals were prepared and distributed into four groups (n = 15) according to the final irrigation protocol: G1, final irrigation with 2.5 mL of distilled water; G2, final irrigation with 2.5 mL of 2.5% sodium hypochlorite solution; G3, final irrigation with 2.5 mL of 17% EDTA; and G4, final irrigation with 2.5 mL of 10% citric acid. Five specimens from each group were not filled to assess smear layer removal by scanning electron microscopy. Ten specimens from each group were filled for analysis of sealer penetration into dentinal tubules by confocal laser scanning microscopy. Smear layer removal (Kruskal-Wallis and Dunn's tests) and sealer penetration (F and Tukey's tests) were statistically analyzed with 95% of significance level. G3 and G4 had greater smear layer removal rates in the cervical and middle thirds, in comparison with G1 and G2 (p < .05). G3 and G4 had the highest percentages of sealer penetration in all thirds, in comparison with G1 and G2 (p < .05). Smear layer removal was effective only at the cervical and middle thirds when the chelating solutions were used. Sealer penetration into the dentinal tubules significantly increased in all root thirds when the specimens were treated with both chelating solutions.
本研究的目的是比较不同螯合溶液（17%乙二胺四乙酸和10%柠檬酸）对玷污层去除的效果及其对根管牙本质封闭剂渗透的影响。制备60个根管，并根据最终冲洗方案分为四组（n = 15）：G1组，用2.5 mL蒸馏水进行最终冲洗；G2组，用2.5 mL 2.5%次氯酸钠溶液进行最终冲洗；G3组，用2.5 mL 17%乙二胺四乙酸进行最终冲洗；G4组，用2.5 mL 10%柠檬酸进行最终冲洗。每组取5个样本不进行充填，通过扫描电子显微镜评估玷污层的去除情况。每组取10个样本进行充填，通过共聚焦激光扫描显微镜分析封闭剂向牙本质小管内的渗透情况。采用Kruskal-Wallis检验和Dunn检验对玷污层去除情况进行统计学分析，采用F检验和Tukey检验对封闭剂渗透情况进行统计学分析，显著性水平为95%。与G1组和G2组相比，G3组和G4组在根管颈段和中段的玷污层去除率更高（p < 0.05）。与G1组和G2组相比，G3组和G4组在所有根管段的封闭剂渗透百分比最高（p < 0.05）。使用螯合溶液时，玷污层去除仅在根管颈段和中段有效。当样本用两种螯合溶液处理时，封闭剂向牙本质小管内的渗透在所有根管段均显著增加。
