Bhatia Simren, Vaidya Mrunalini, Hegde Vibha, Sachdev Sanpreet Singh
Department of Conservative Dentistry and Endodontics, Dr. G. D. Pol Foundation's YMT Dental College, Navi Mumbai, India.
Department of Oral Pathology and Microbiology, Bharati Vidyapeeth (Deemed to be University) Dental College and Hospital, Navi Mumbai, India.
Eur Endod J. 2025 Mar;10(2):173-180. doi: 10.14744/eej.2024.30092.
Sodium hypochlorite is a common irrigant in endodontics, used to eliminate microorganisms and dissolve pulpal tissue. However, adjunctive chelating agents, while aiding in smear layer removal, can reduce dentinal wall microhardness, affecting dentin permeability, solubility, and the sealing ability of root canal sealers. This study aims to evaluate the impact of newer chelating agents-Silver Citrate, Chitosan, HEBP, and EDTA-on the mineral composition of root canal dentin using energy-dispersive X-ray Spectroscopy (EDXS).
The root canals of 120 freshly extracted human mandibular premolars with single straight canals and intact, mature apices were prepared following standard endodontic procedures using Rotary Pro Taper nickel-titanium files. Final irrigation was conducted with 5 mL of the respective chelating solution. Changes in the mineral composition of the root canal dentin were analyzed using EDXS. Descriptive statistics were presented as means and standard deviations. The impact of chelating solutions on the mineral content of root canal dentin at the cervical, middle, and apical thirds was compared between groups using One-Way ANOVA followed by a post hoc Bonferroni test.
The highest mean concentrations of minerals such as Mg, Ca, and P at both the coronal and apical levels (p<0.05), along with the Ca/P ratio in the coronal third, were observed with distilled water, followed by HEBP, Chitosan, EDTA, and Silver Citrate, which showed the lowest values. In the middle third, the concentrations of Ca and P, as well as the Ca/P ratio in the apical and middle thirds, followed the sequence: Distilled Water>EDTA>HEBP>Chitosan>Silver Citrate. The difference in the levels of all minerals was statistically significant (p<0.05) at the coronal, middle and apical third levels.
Among the tested solutions, 9% HEBP caused the least alteration in the mineral content of root canal dentin when compared to 0.2% Chitosan, 17% EDTA, and Silver Citrate. This suggests that HEBP may be a preferable choice in endodontic procedures where minimal alteration of dentin mineral content is desired. (EEJ-2024-08-124).
次氯酸钠是牙髓病治疗中常用的冲洗剂，用于消除微生物和溶解牙髓组织。然而，辅助螯合剂在帮助去除玷污层的同时，会降低牙本质壁的显微硬度，影响牙本质通透性、溶解性以及根管封闭剂的封闭能力。本研究旨在使用能量色散X射线光谱法（EDXS）评估新型螯合剂——柠檬酸银、壳聚糖、1-羟基亚乙基二膦酸（HEBP）和乙二胺四乙酸（EDTA）对根管牙本质矿物质组成的影响。
选取120颗新鲜拔除的具有单一直根管且根尖完整、成熟的人下颌前磨牙，按照标准牙髓病治疗程序，使用旋转Pro Taper镍钛锉预备根管。用5 mL相应的螯合溶液进行最终冲洗。使用EDXS分析根管牙本质矿物质组成的变化。描述性统计数据以均值和标准差表示。采用单因素方差分析，随后进行事后Bonferroni检验，比较各螯合溶液组对根管牙本质颈段、中段和根尖段矿物质含量的影响。
在冠部和根尖水平，蒸馏水组观察到Mg、Ca和P等矿物质的平均浓度最高（p<0.05），同时冠部三分之一处的Ca/P比值也最高，其次是HEBP、壳聚糖、EDTA和柠檬酸银，柠檬酸银组的数值最低。在中段，Ca和P的浓度以及根尖段和中段的Ca/P比值顺序为：蒸馏水>EDTA>HEBP>壳聚糖>柠檬酸银。在冠部、中段和根尖段水平，所有矿物质含量水平的差异均具有统计学意义（p<0.05）。
与0.2%壳聚糖、17%EDTA和柠檬酸银相比，在测试溶液中，9%HEBP对根管牙本质矿物质含量的改变最小。这表明，在希望对牙本质矿物质含量进行最小改变的牙髓病治疗程序中，HEBP可能是更优选择。（EEJ - 2024 - 08 - 124）
