Department of Endodontics, School of Stomatology, China Medical University, Shenyang, Liaoning 110002, P.R. China.
Department of Stem Cells and Regenerative Medicine, Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang, Liaoning 110013, P.R. China.
Int J Mol Med. 2018 Feb;41(2):791-799. doi: 10.3892/ijmm.2017.3261. Epub 2017 Nov 17.
Since the beginning of the use of stem cells in tissue regenerative medicine, there has been a search for optimal sources of stem cells. Human amniotic epithelial cells (hAECs) are derived from human amnions, which are typically discarded as medical waste, but were recently found to include cells with trilineage differentiation potential in vitro. Previous study has focused on the osteogenic differentiation ability of hAECs as seed cells in bone regeneration; however, their paracrine effects on osteoblasts (OBs) are yet to be elucidated. In the present study, conditioned medium (CM) derived from hAECs was used to determine their paracrine effects on the human fetal OB cell line (hFOB1.19), and the potential bioactive factors involved in this process were investigated. The results suggested that hAEC-CM markedly promoted the proliferation, migration and osteogenic differentiation of hFOB1.19 cells. Expression of transforming growth factor β1 (TGFβ1) and microRNA 34a-5p (miR-34a-5p) were detected in hAECs. Furthermore, it was demonstrated that TGFβ1 and miR-34a-5p stimulated the differentiation of hFOB1.19 cells, and that TGFβ1 promoted cell migration. Moreover, the effects of hAEC-CM were downregulated following the depletion of either TGFβ1 or miR-34a-5p. These results demonstrated that hAECs promote OB differentiation through the secretion of TGFβ1 and miR-34a-5p, and that hAECs may be an optimal cell source in bone regenerative medicine.
自干细胞在组织再生医学中的应用开始以来，人们一直在寻找最佳的干细胞来源。人羊膜上皮细胞（hAECs）来源于人羊膜，通常作为医疗废物丢弃，但最近发现其在体外具有三系分化潜能。先前的研究集中在 hAECs 作为种子细胞在骨再生中的成骨分化能力上；然而，其对成骨细胞（OBs）的旁分泌作用尚未阐明。在本研究中，使用源自 hAECs 的条件培养基（CM）来确定其对人胎儿 OB 细胞系（hFOB1.19）的旁分泌作用，并研究了涉及该过程的潜在生物活性因子。结果表明，hAEC-CM 显著促进了 hFOB1.19 细胞的增殖、迁移和成骨分化。在 hAECs 中检测到转化生长因子β1（TGFβ1）和 microRNA 34a-5p（miR-34a-5p）的表达。此外，证明 TGFβ1 和 miR-34a-5p 刺激 hFOB1.19 细胞分化，并且 TGFβ1 促进细胞迁移。此外，在耗尽 TGFβ1 或 miR-34a-5p 后，hAEC-CM 的作用被下调。这些结果表明，hAECs 通过分泌 TGFβ1 和 miR-34a-5p 促进 OB 分化，并且 hAECs 可能是骨再生医学中的最佳细胞来源。
