Department of Oral and Craniomaxillofacial Science, Ninth People's Hospital College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China.
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Stem Cells Int. 2015;2015:565732. doi: 10.1155/2015/565732. Epub 2015 Mar 5.
Emerging evidence suggests amniotic epithelial cells (AECs) as a promising source of progenitor cells in regenerative medicine and bone tissue engineering. However, investigations comparing the regenerative properties of AECs with other sources of stem cells are particularly needed before the feasibility of AECs in bone tissue engineering can be determined. This study aimed to compare human amniotic epithelial cells (hAECs), human bone marrow mesenchymal stem cells (hBMSCs), and human amniotic fluid derived mesenchymal stem cells (hAFMSCs) in terms of their morphology, proliferation, immunophenotype profile, and osteogenic capacity in vitro and in vivo. Not only greatly distinguished by cell morphology and proliferation, hAECs, hAFMSCs, and hBMSCs exhibited remarkably different signature regarding immunophenotypical profile. Microarray analysis revealed a different expression profile of genes involved in ossification along the three cell sources, highlighting the impact of different anatomical origin and molecular response to osteogenic induction on the final tissue-forming potential. Furthermore, our data indicated a potential role of FOXC2 in early osteogenic commitment.
新出现的证据表明，羊膜上皮细胞（AECs）是再生医学和骨组织工程中祖细胞的有前途的来源。然而，在确定 AEC 在骨组织工程中的可行性之前，特别需要比较 AEC 与其他干细胞来源的再生特性。本研究旨在比较人羊膜上皮细胞（hAECs）、人骨髓间充质干细胞（hBMSCs）和人羊膜来源的间充质干细胞（hAFMSCs）在体外和体内的形态、增殖、免疫表型特征和成骨能力。hAECs、hAFMSCs 和 hBMSCs 不仅在细胞形态和增殖方面有很大的区别，而且在免疫表型特征方面也有明显不同的特征。微阵列分析显示，三种细胞来源的成骨相关基因表达谱存在差异，突出了不同解剖起源和对成骨诱导的分子反应对最终组织形成潜力的影响。此外，我们的数据表明 FOXC2 在早期成骨定向中可能发挥作用。
