Deussen A, Pau H
Zentrum für Physiologie und Klin. Physiologie, Universität Düsseldorf, West Germany.
Exp Eye Res. 1989 Jan;48(1):37-47. doi: 10.1016/0014-4835(89)90016-x.
Sensitive high-performance liquid chromatography methods were employed to assess regional distribution of adenine, guanosine and uridine nucleotides in clear and cataractous human eye lenses. According to slit-lamp examination, three forms of senile cataract were distinguished: (1) supranuclear or deep cortical cataract (typical senile cataract), (2) primary nuclear cataract (cataracta brunescens) and (3) subcapsular cortical cataract associated either with a supranuclear (3a) or a secondary nuclear cataract (3b). Except for AMP, which was highest in the nuclear fraction, all other nucleotides (ATP, ADP, GTP, and UTP) were predominantly located in the anterior cortex (plus epithelium) of clear as well as cataractous lenses, that is, ATP levels in the nucleus amounted to 20% of those found in the anterior cortex (plus epithelium); ATP levels in the posterior cortex were about 60% of those in the anterior cortex (plus epithelium). Significant differences in the absolute regional nucleotide level existed between the different forms of cataract. Highest ATP levels were found in the anterior cortex (plus epithelium) of clear lenses and deep or supranuclear cortical cataract. The ATP level was slightly diminished in primary nuclear cataract and in supranuclear cortical cataract when associated with an early subcapsular cortical cataract. ATP levels were depressed to less than 30% in the anterior cortex (plus epithelium) of lenses with a subcapsular cortical cataract when associated with either an early secondary nuclear or a mature cataract. Furthermore, the ATP/ADP ratio was decreased in this form of senile cataract. The decrease in lens nucleotide level did not correlate with increased age. These data suggest that decreases in regional ATP level are a secondary event and do not appear to be causally involved in the genesis of the 'cataracta senilis'.
采用灵敏的高效液相色谱法评估腺嘌呤、鸟苷和尿苷核苷酸在透明和白内障人眼晶状体中的区域分布。根据裂隙灯检查，区分出三种老年性白内障：（1）核上或深层皮质性白内障（典型老年性白内障），（2）原发性核性白内障（棕色白内障）和（3）与核上性（3a）或继发性核性白内障（3b）相关的囊下皮质性白内障。除了在核部分中含量最高的AMP外，所有其他核苷酸（ATP、ADP、GTP和UTP）主要位于透明以及白内障晶状体的前皮质（加上皮）中，也就是说，核中的ATP水平相当于前皮质（加上皮）中ATP水平的20%；后皮质中的ATP水平约为前皮质（加上皮）中ATP水平的60%。不同类型白内障之间区域核苷酸绝对水平存在显著差异。透明晶状体以及深层或核上皮质性白内障的前皮质（加上皮）中ATP水平最高。原发性核性白内障以及与早期囊下皮质性白内障相关的核上皮质性白内障中，ATP水平略有降低。当与早期继发性核性或成熟白内障相关时，囊下皮质性白内障晶状体的前皮质（加上皮）中ATP水平降至不到30%。此外，这种老年性白内障中ATP/ADP比值降低。晶状体核苷酸水平的降低与年龄增加无关。这些数据表明，区域ATP水平的降低是继发事件，似乎与“老年性白内障”的发生没有因果关系。
