西非冈比亚发生的与柯萨奇病毒A24变种相关的急性出血性结膜炎疫情。
An outbreak of acute haemorrhagic conjunctivitis associated with coxsackievirus A24 variant in The Gambia, West Africa.
作者信息
Burr Sarah E, Sillah Ansumana, Joof Hassan, Bailey Robin L, Holland Martin J
机构信息
Clinical Research Department, London School of Hygiene and Tropical Medicine, London, UK.
Disease Control and Elimination Theme, Medical Research Council Unit, The Gambia, Fajara, Banjul, The Gambia.
出版信息
BMC Res Notes. 2017 Dec 6;10(1):692. doi: 10.1186/s13104-017-3007-9.
OBJECTIVE
An outbreak of acute haemorrhagic conjunctivitis occurred in The Gambia, West Africa in 2011. Affected individuals presented with conjunctival haemorrhages, swelling and ocular discharge. In an effort to identify a causative agent of the disease, ocular swabs were taken from patients during the acute and convalescent phases. Total RNA was extracted from all samples and reverse-transcriptase PCR performed using primers specific for all enteroviruses. Resulting amplicons were sequenced and data compared to known sequences using the BLAST algorithm.
RESULTS
Forty-eight swabs were included in the analysis. Of these, 21 acute and 9 convalescent swabs (65% of the total) gave positive PCR results. Sequence analysis of the resulting amplicons indicated 99% sequence identity with coxsackievirus A24 variant identified during independent outbreaks of acute haemorrhagic conjunctivitis around the world and suggest the Gambian outbreak was due to this virus.
目的
2011年，西非的冈比亚爆发了急性出血性结膜炎。受影响的个体出现结膜出血、肿胀和眼部分泌物。为了确定该疾病的病原体，在急性期和恢复期从患者身上采集了眼拭子。从所有样本中提取总RNA，并使用针对所有肠道病毒的引物进行逆转录聚合酶链反应。对产生的扩增子进行测序，并使用BLAST算法将数据与已知序列进行比较。
结果
48份拭子纳入分析。其中，21份急性期拭子和9份恢复期拭子（占总数的65%）PCR结果呈阳性。对产生的扩增子进行序列分析表明，其与在世界各地急性出血性结膜炎独立暴发期间鉴定出的柯萨奇病毒A24变种有99%的序列同一性，提示冈比亚的暴发是由该病毒引起的。
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