用微焦点计算机断层扫描对人胎盘微循环成像：组织准备和图像采集的优化。
Imaging the human placental microcirculation with micro-focus computed tomography: Optimisation of tissue preparation and image acquisition.
机构信息
Translational Imaging Group, Dept. Medical Physics and Biomedical Engineering, University College London, Malet Place Engineering Building, Gower Street, London WC1E 6BT, UK; Institute for Women's Health, University College London, 86-96 Chenies Mews, London WC1E 6HX, UK.
University College London Great Ormond Street Institute of Child Health, University College London, 30 Guilford Street, London, WC1N 1EH, UK; Department of Histopathology, Great Ormond Street Hospital for Children NHS Trust, Great Ormond Street, London, WC1N 3JH London, UK.
出版信息
Placenta. 2017 Dec;60:36-39. doi: 10.1016/j.placenta.2017.09.013. Epub 2017 Oct 3.
Micro-CT provides 3D volume imaging with spatial resolution at the micrometre scale. We investigated the optimal human placenta tissue preparation (contrast agent, perfusion pressure, perfusion location and perfusion vessel) and imaging (energy, target material, exposure time and frames) parameters. Microfil (Flow Tech, Carver, MA) produced better fill than Barium sulphate (84.1%(±11.5%)vs70.4%(±18.02%) p = 0.01). Perfusion via umbilical artery produced better fill than via chorionic vessels (83.8%(±17.7%)vs78.0%(±21.9%), p < 0.05), or via umbilical vein (83.8%(±16.4%)vs69.8%(±20.3%), p < 0.01). Imaging at 50 keV with a molybdenum target produced the best contrast to noise ratio. We propose this method to enable quantification and comparison of the human fetoplacental vascular tree.
微计算机断层扫描（Micro-CT）提供具有微米级空间分辨率的 3D 容积成像。我们研究了最佳的人胎盘组织制备（对比剂、灌注压力、灌注位置和灌注血管）和成像（能量、靶材料、曝光时间和帧数）参数。微丝（FlowTech，马萨诸塞州卡弗）产生的充盈效果优于硫酸钡（84.1%（±11.5%）对 70.4%（±18.02%），p=0.01）。通过脐动脉灌注比通过绒毛血管（83.8%（±17.7%）对 78.0%（±21.9%），p<0.05）或通过脐静脉（83.8%（±16.4%）对 69.8%（±20.3%），p<0.01）灌注产生更好的充盈效果。用钼靶进行 50keV 成像可获得最佳的对比噪声比。我们提出这种方法来实现对人胎-胎盘血管树的定量和比较。
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