Liu Xiaobing, Terry Toya, Pan Su, Yang Zhongwei, Willerson James T, Dixon Richard A F, Liu Qi
The Texas Heart Institute at St. Luke's Episcopal Hospital, TX, USA.
J Vis Exp. 2013 Jun 29(76):50364. doi: 10.3791/50364.
Preclinical research in animal models of peripheral arterial disease plays a vital role in testing the efficacy of therapeutic agents designed to stimulate microcirculation. The choice of delivery method for these agents is important because the route of administration profoundly affects the bioactivity and efficacy of these agents(1,2). In this article, we demonstrate how to locally administer a substance in ischemic hindlimbs by using a catheterized osmotic pump. This pump can deliver a fixed volume of aqueous solution continuously for an allotted period of time. We also present our mouse model of unilateral hindlimb ischemia induced by ligation of the common femoral artery proximal to the origin of profunda femoris and epigastrica arteries in the left hindlimb. Lastly, we describe the in vivo cannulation and ligation of the infrarenal abdominal aorta and perfusion of the hindlimb vasculature with Microfil, a silicone radiopaque casting agent. Microfil can perfuse and fill the entire vascular bed (arterial and venous), and because we have ligated the major vascular conduit for exit, the agent can be retained in the vasculature for future ex vivo imaging with the use of small specimen micro-CT(3).
外周动脉疾病动物模型的临床前研究在测试旨在刺激微循环的治疗药物的疗效方面发挥着至关重要的作用。这些药物给药方法的选择很重要，因为给药途径会深刻影响这些药物的生物活性和疗效(1,2)。在本文中，我们展示了如何通过使用导管插入式渗透泵在缺血后肢局部给药一种物质。该泵可以在规定的时间段内持续输送固定体积的水溶液。我们还展示了我们通过结扎左后肢股深动脉和腹壁动脉起始处近端的股总动脉诱导单侧后肢缺血的小鼠模型。最后，我们描述了肾下腹主动脉的体内插管和结扎以及用一种硅酮不透射线铸型剂Microfil对后肢血管系统进行灌注。Microfil可以灌注并填充整个血管床（动脉和静脉），并且由于我们已经结扎了主要的血管流出通道，该试剂可以保留在血管系统中，以便将来使用小型标本微型CT进行离体成像(3)。
