Department of Physics, Guru Nanak Dev University, Amritsar 143005, India.
Department of Physics, Guru Nanak Dev University, Amritsar 143005, India.
Mater Sci Eng C Mater Biol Appl. 2018 Feb 1;83:177-186. doi: 10.1016/j.msec.2017.09.006. Epub 2017 Sep 29.
A diopside based bioactive system with a nominal composition of xCuO-(45.55-x)CaO-29.44 SiO-10.28PO-14.73 MgO (x=0,1,3 and 5mol%) has been prepared by sol gel technique in the laboratory. X-ray Diffraction, Fourier Transform Infra-Red and Raman Spectroscopy, Field Emission Scanning Electron Microscopy along with Energy Dispersive X-ray Analysis and pH studies have been undertaken on the prepared samples before and after dipping the samples in simulated body fluid. It has been observed that hydroxyapatite layer starts to form with in 24h during immersion in simulated body fluid. Degradation studies have also been employed to check the degradation behavior in Tris-HCl. Dynamic light scattering studies show that particles are mostly agglomerated and have an average size of 356nm. Zeta potential studies have been undertaken to check the surface charge and it has been estimated that samples carry negative charge when dipped in simulated body fluid. Negative surface charge may contribute to attachment and proliferation of osteoblasts. Samples have also shown the antimicrobial properties against the Vibro cholerae and Escherichia coli pathogens. To check the non-toxic nature of the samples, cell cytotoxic and cell culture studies have been undertaken using the MG-63 cell lines. Samples have shown good response with good percentage viability of the cells in the culture media and hence, provides friendly environment to the growth of cells. The particle size, bioactivity, negative values of zeta potential, antimicrobial properties and good cell viability indicate the potential of the synthesized compositions as possible candidates for bone repair applications.
采用溶胶-凝胶技术在实验室中制备了一种以透辉石为基础的生物活性系统，其名义组成为 xCuO-(45.55-x)CaO-29.44SiO-10.28PO-14.73MgO（x=0、1、3 和 5mol%）。在将样品浸入模拟体液前后，对制备的样品进行了 X 射线衍射、傅里叶变换红外和拉曼光谱、场发射扫描电子显微镜以及能量色散 X 射线分析和 pH 值研究。观察到在浸入模拟体液后 24 小时内开始形成羟基磷灰石层。还进行了降解研究以检查在 Tris-HCl 中的降解行为。动态光散射研究表明，颗粒主要聚集并且平均粒径为 356nm。进行了 Zeta 电位研究以检查表面电荷，并且当样品浸入模拟体液中时，估计其带有负电荷。负表面电荷可能有助于成骨细胞的附着和增殖。样品还显示出对霍乱弧菌和大肠杆菌病原体的抗菌性能。为了检查样品的非毒性性质，使用 MG-63 细胞系进行了细胞毒性和细胞培养研究。样品在培养基中显示出良好的细胞活力百分比，表现出良好的反应，因此为细胞的生长提供了友好的环境。颗粒尺寸、生物活性、Zeta 电位的负值、抗菌性能和良好的细胞活力表明合成组合物作为骨修复应用的潜在候选物的潜力。
