Borg B, Schoonen W G, Lambert J G
Department of Zoology, University of Stockholm, Sweden.
Gen Comp Endocrinol. 1989 Jan;73(1):40-5. doi: 10.1016/0016-6480(89)90053-1.
Steroid metabolism in the testes of sticklebacks was studied in vitro by tissue incubations with [3H]pregnenolone or [3H]androstenedione as precursors. In males in full reproductive condition (nesting), [3H]pregnenolone was mainly converted via progesterone and 17 alpha-hydroxyprogesterone into androstenedione, 11 beta-hydroxyandrostenedione, and 11-ketoandrostenedione. The latter was the largest product formed. The main products from the [3H]androstenedione incubation, 11 beta-hydroxyandrostenedione and 11-ketoandrostenedione, confirm these findings. The rate of androgen synthesis, especially of 11-ketoandrostenedione, was much lower after the end of the breeding season.
通过用[3H]孕烯醇酮或[3H]雄烯二酮作为前体进行组织培养，在体外研究了刺鱼睾丸中的类固醇代谢。在处于完全生殖状态（筑巢）的雄性刺鱼中，[3H]孕烯醇酮主要通过孕酮和17α-羟孕酮转化为雄烯二酮、11β-羟基雄烯二酮和11-酮基雄烯二酮。后者是形成的最大产物。[3H]雄烯二酮培养的主要产物11β-羟基雄烯二酮和11-酮基雄烯二酮证实了这些发现。繁殖季节结束后，雄激素合成速率，尤其是11-酮基雄烯二酮的合成速率，要低得多。
