Haro Cecilia, Mónaco María E, Medina Marcela
Instituto de Bioquímica Aplicada, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas).
Instituto de Biología, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán.
Blood Coagul Fibrinolysis. 2018 Jan;29(1):104-110. doi: 10.1097/MBC.0000000000000684.
: The current study aims at evaluating the effect of the oral administration of Lactobacillus casei CERELA (CRL) 431 on parameters implicated in inflammation-coagulation interaction using a model of acute inflammation induced by lipopolysaccharide (LPS) in mice. Six-week-old Balb/c mice were treated with L. casei for 5 consecutive days. Then treated and untreated mice received an LPS injection (L. casei + LPS and LPS groups, respectively). Liver and kidney were removed, blood samples were obtained, and hemostatic and inflammatory parameters were evaluated at different times post LPS injection. Preventive L. casei administration induced a significant decrease in proinflammatory TNF-α and IL-6 cytokines by decreasing tissue factor expression in liver and kidney. Moreover, the lower expression of tissue factor in the L. casei + LPS group led to a lower activation of the coagulation system, which was observed by the fast systemic restoration of factors VII and V coagulation factors and antithrombin levels. This study highlights the capacity of L. casei to modulate the hemostatic unbalance in an acute endotoxemia model. Our findings showed the ability of L. casei CRL 431 to regulate the immuno-coagulative response. This fact could be helpful to propose new adjunctive strategies addressed to the restoration of physiological anticoagulant mechanisms in sepsis patients.
本研究旨在利用小鼠脂多糖（LPS）诱导的急性炎症模型，评估口服干酪乳杆菌CERELA（CRL）431对炎症 - 凝血相互作用相关参数的影响。六周龄的Balb/c小鼠连续5天接受干酪乳杆菌治疗。然后，治疗组和未治疗组小鼠分别接受LPS注射（分别为干酪乳杆菌+LPS组和LPS组）。在注射LPS后的不同时间点，取出肝脏和肾脏，采集血样，并评估止血和炎症参数。预防性给予干酪乳杆菌可通过降低肝脏和肾脏中组织因子的表达，显著降低促炎细胞因子TNF-α和IL-6。此外，干酪乳杆菌+LPS组中组织因子的较低表达导致凝血系统的激活程度较低，这可通过凝血因子VII和V以及抗凝血酶水平的快速全身恢复观察到。本研究强调了干酪乳杆菌在急性内毒素血症模型中调节止血失衡的能力。我们的研究结果显示了干酪乳杆菌CRL 431调节免疫凝血反应的能力。这一事实可能有助于提出新的辅助策略，以恢复脓毒症患者的生理抗凝机制。
