Portilho Moyra Machado, Nabuco Leticia Cancella, Villela-Nogueira Cristiane Alves, Brandão-Mello Carlos Eduardo, Pilotto José Henrique, Flores Geane Lopes, Lewis-Ximenez Lia Laura, Lampe Elisabeth, Villar Livia Melo
Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Hepatites Virais, Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro, Hospital Universitário Clementino Fraga Filho, Rio de Janeiro, RJ, Brasil.
Mem Inst Oswaldo Cruz. 2018 Jan;113(1):62-65. doi: 10.1590/0074-02760170071.
In occult hepatitis B infection (OBI), hepatitis B virus DNA (HBV DNA) can be detected in serum samples; however, oral fluid collection for detection of HBV DNA has not yet been explored, despite the availability of collection devices. Serum and oral fluid samples from 45 hepatitis B core antibody (anti-HBc)-positive patients were collected for the amplification of the HBV polymerase gene. HBV DNA was detected in five serum and four oral fluid samples (the detection limit for oral fluid was 1.656 log IU/mL in paired serum). In conclusion, simple methodologies of sample collection and in-house polymerase chain reaction (PCR) allowed detection of HBV DNA, and these could be used to improve the diagnosis of OBI, especially in locations with limited resources.
在隐匿性乙型肝炎感染（OBI）中，血清样本中可检测到乙型肝炎病毒DNA（HBV DNA）；然而，尽管有采集装置，但尚未探索过采集口腔液来检测HBV DNA。收集了45例乙型肝炎核心抗体（抗-HBc）阳性患者的血清和口腔液样本，用于扩增HBV聚合酶基因。在5份血清样本和4份口腔液样本中检测到了HBV DNA（配对血清中口腔液的检测限为1.656 log IU/mL）。总之，简单的样本采集方法和内部聚合酶链反应（PCR）能够检测到HBV DNA，这些方法可用于改善OBI的诊断，尤其是在资源有限的地区。
