Laboratory of Viral Hepatitis, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil.
Federal Institute of Education, Science and Technology of Ceará, Campus Fortaleza, Ceará, Brazil.
Can J Gastroenterol Hepatol. 2019 Apr 1;2019:5672795. doi: 10.1155/2019/5672795. eCollection 2019.
Hepatitis B virus (HBV) is one of the major causes of chronic liver disease worldwide; however most of individuals are not aware about the infection. Oral fluid and dried blood spot (DBS) samples may be an alternative to serum to HBV diagnosis to increase the access to diagnosis in remote areas or high-risk groups. The main objective of this review is to give an insight about the usefulness of oral fluid and DBS for detecting HBV markers. Several groups have evaluated the detection of HBsAg, anti-HBc, and anti-HBs markers in oral fluid and DBS samples demonstrating 13 to 100% of sensitivity and specificity according different groups, sample collectors, and diagnosis assays. In the same way, HBV DNA detection using oral fluid and DBS samples demonstrate different values of sensitivity according type of collection, studied group, extraction, and detection methods. Thus, serological and molecular diagnostic tests demonstrated good performance for detecting HBV using oral fluid and DBS according some characteristics and could be useful to increase the access to the diagnosis of HBV.
乙型肝炎病毒（HBV）是全球慢性肝病的主要病因之一；然而，大多数人并不知道自己已感染。与血清相比，唾液和干血斑（DBS）样本可能是乙型肝炎病毒诊断的另一种选择，可以增加偏远地区或高危人群获得诊断的机会。本综述的主要目的是深入了解唾液和 DBS 样本在检测乙型肝炎病毒标志物方面的作用。有几个研究小组评估了唾液和 DBS 样本中 HBsAg、抗-HBc 和抗-HBs 标志物的检测，根据不同的研究对象、样本采集者和诊断检测方法，其灵敏度和特异性分别为 13%至 100%。同样，使用唾液和 DBS 样本检测 HBV DNA 的灵敏度也因采集方式、研究对象、提取和检测方法的不同而有所不同。因此，根据某些特征，血清学和分子诊断检测在使用唾液和 DBS 检测 HBV 方面表现出良好的性能，可能有助于增加乙型肝炎病毒的诊断机会。
