Marczuk-Kolada Grażyna, Łuczaj-Cepowicz Elżbieta, Pawińska Małgorzata, Hołownia Adam
Department of Pedodontics, Medical University of Bialystok, Poland.
Department of Conservative Dentistry, Medical University of Bialystok, Poland.
Adv Clin Exp Med. 2017 Oct;26(7):1041-1045. doi: 10.17219/acem/64944.
Dentistry materials are the most frequently used substitutes of human tissues. Therefore, an assessment of dental filling materials should cover not only their chemical, physical, and mechanical characteristics, but also their cytotoxicity.
To compare the cytotoxic effects of 13 conventional glass ionomer cements on human gingival fibroblasts.
The assessment was conducted using the MTT test. Six samples were prepared for each material. Culture plates with cells and inserts with the materials were incubated at 37°C, 5% CO2, and 95% humidity for 24 h. Then the inserts were removed, 1 mL of MTT was added in the amount of 0.5 mg/1 mL of the medium, and the samples were incubated in the described conditions without light for 2 h. The optical density was measured with an absorption spectrophotometer at a wavelength of 560 nm.
The cytotoxic effects of the Argion Molar was significantly stronger than the Fuji Triage (p = 0.007), Chemfil Molar (p < 0.0001), and Ionofil Molar AC Quick (p < 0.001). The Fuji IX GP and Fuji IX Extra had a significantly stronger adverse effect than the Chemfil Molar (p = 0.014, p = 0.029, respectively) and Ionofil Molar AC Quick (p = 0.017, p = 0.034, respectively). The cements from the low cytotoxicity group were significantly more toxic vs materials whose presence resulted in fibroblast growth (p < 0.001).
The research conducted indicates that, although the materials studied may belong to the same group, they are characterized by low, yet not uniform, cytotoxicity on human gingival fibroblasts. The toxic effects should not be assigned to a relevant group of materials, but each dentistry product should be evaluated individually.
牙科材料是最常用的人体组织替代物。因此，对牙科填充材料的评估不仅应涵盖其化学、物理和机械特性，还应包括其细胞毒性。
比较13种传统玻璃离子水门汀对人牙龈成纤维细胞的细胞毒性作用。
采用MTT试验进行评估。每种材料制备6个样本。将带有细胞的培养板和带有材料的插入物在37°C、5%二氧化碳和95%湿度条件下孵育24小时。然后取出插入物，以0.5mg/1mL培养基的量加入1mL MTT，样本在上述无光条件下孵育2小时。用吸收分光光度计在560nm波长处测量光密度。
Argion Molar的细胞毒性作用明显强于Fuji Triage（p = 0.007）、Chemfil Molar（p < 0.0001）和Ionofil Molar AC Quick（p < 0.001）。Fuji IX GP和Fuji IX Extra的不良作用明显强于Chemfil Molar（分别为p = 0.014，p = 0.029）和Ionofil Molar AC Quick（分别为p = 0.017，p = 0.034）。低细胞毒性组的水门汀与能促进成纤维细胞生长的材料相比，毒性明显更高（p < 0.001）。
所进行的研究表明，尽管所研究的材料可能属于同一组，但它们对人牙龈成纤维细胞的细胞毒性较低但并不一致。不应将毒性作用归因于某一相关材料组，而应单独评估每种牙科产品。
