Cao S L, Liu T F, Wang Z H, Zhou J, Huang K M, Yao W Q, Shen X L
Department of Radiation Oncology, Cancer Hospital and Institute, Shanghai Medical University, China.
Int J Radiat Oncol Biol Phys. 1989 Feb;16(2):343-5.
The tissue from a patient with nasopharyngeal carcinoma has been transferred to nude mice (BALB/c), and has successfully been growing through twenty passages. The tumors in the nude mice and primary human tumor have been examined for the cellular DNA content by FCM and also conventional pathological examination, chromosome analysis, and EBV test. The tumor take rate varied markedly in different passages with a mean value of approximately 70%, and showing a tendency to increase. The tumor doubling time within 6-12 weeks after transplantation of six tumors in 18 and 20 passages were 14.8 and 9.3 days respectively. However, the tumor volume at 12 weeks varied significantly, ranging from 438 to 1998 mm3. By FCM, it has been found that the values of DNA index were about the same in both primary tumor and the tumors in nude mice. The distribution of various phase cells in cell cycle was also about the same in both. In conclusion, the application of FCM to examine the cellular DNA content of the tumor in nude mice is a rapid and sensitive method, useful in the investigation on the stability of biological characteristics of human NPC in nude mice and in further studies on the effects by various therapeutic methods.
一名鼻咽癌患者的组织已被移植到裸鼠（BALB/c）体内，并已成功传代20次。通过流式细胞术（FCM）以及传统病理检查、染色体分析和EB病毒检测，对裸鼠体内的肿瘤和原发性人类肿瘤的细胞DNA含量进行了检测。不同传代的肿瘤接种率差异显著，平均值约为70%，且呈上升趋势。在第18代和第20代移植的6个肿瘤中，移植后6 - 12周内肿瘤的倍增时间分别为14.8天和9.3天。然而，12周时肿瘤体积差异显著，范围为438至1998立方毫米。通过流式细胞术发现，原发性肿瘤和裸鼠体内肿瘤的DNA指数值大致相同。两者细胞周期中各期细胞的分布也大致相同。总之，应用流式细胞术检测裸鼠体内肿瘤的细胞DNA含量是一种快速、灵敏的方法，有助于研究人类鼻咽癌在裸鼠体内生物学特性的稳定性以及各种治疗方法的效果。
