Cao S L, Liu T F, Wang Z H, Huang K M, Zhou J, Li D L, Ni H Z, Sang J Z, Wang Y, Zhang B N
Zhonghua Zhong Liu Za Zhi. 1987 Jan;9(1):6-9.
Tumor tissue from a patient with nasopharyngeal carcinoma was transplanted to nude mice (BALB/c) and had successfully been maintained through ten passages. The nude mice tumor and the primary human tumor were assayed by FCM for the DNA content in addition to conventional pathological examination, chromosome analysis and EBV assay. The tumor incidence in the different passages showed a marked change ranging from 25% to 81% with a mean value of 52% which tended to increase, however. The doubling time of six tumors in the ninth passage showed no great change 6-12 weeks after transplantation with a mean about 4.3 days. But at week 12, there was a significant difference between each tumor volume, ranging from 438 to 1,998 mm3. By FCM, it was found that the DNA index remained constant in both the primary and nude mice tumors. Among the nude mice tumors in different passages, the distribution of various phase cells in cell cycle was similar to that of the primary tumor. In conclusion, the use of FCM to assay the cellular DNA content in nude mice tumor is rapid and sensitive. It is helpful, for the nude mice tumor, in identifying stability of the biological characteristics and in studying of the mechanism of chemotherapy and radiotherapy in the future.
将一名鼻咽癌患者的肿瘤组织移植到裸鼠（BALB/c）体内，并成功传代了十次。除了进行常规病理检查、染色体分析和EBV检测外，还通过流式细胞术（FCM）对裸鼠肿瘤和原发性人类肿瘤的DNA含量进行了检测。不同传代的肿瘤发生率有显著变化，范围从25%到81%，平均值为52%，不过有上升趋势。第九代的六个肿瘤移植后6 - 12周的倍增时间变化不大，平均约为4.3天。但在第12周时，每个肿瘤体积之间存在显著差异，范围从438到1998立方毫米。通过FCM发现，原发性肿瘤和裸鼠肿瘤的DNA指数均保持恒定。在不同传代的裸鼠肿瘤中，细胞周期中各期细胞的分布与原发性肿瘤相似。总之，使用FCM检测裸鼠肿瘤细胞的DNA含量快速且灵敏。这有助于确定裸鼠肿瘤生物学特性的稳定性，并为未来研究化疗和放疗机制提供帮助。
