Friedemann Thomas, Otto Benjamin, Klätschke Kristin, Schumacher Udo, Tao Yi, Leung Alexander Kai-Man, Efferth Thomas, Schröder Sven
HanseMerkur Center for Traditional Chinese Medicine at the University Clinic Hamburg-Eppendorf, Martinistr. 52, Hamburg 20246, Germany.
Array Service Center, University Medical Center Hamburg-Eppendorf, Martinistr. 52, Hamburg 20246, Germany.
J Ethnopharmacol. 2014 Aug 8;155(1):607-15. doi: 10.1016/j.jep.2014.06.004. Epub 2014 Jun 12.
The dried rhizome of Coptis chinensis Franch. (family Ranunculaceae) is traditionally used in Chinese medicine for the treatment of inflammatory diseases and diabetes. Recent studies showed a variety of activities of Coptis chinensis Franch. alkaloids, including neuroprotective, neuroregenerative, anti-diabetic, anti-oxidative and anti-inflammatory effects. However, there is no report on the neuroprotective effect of Coptis chinensis Franch. watery extract against tert-butylhydroperoxide (t-BOOH) induced oxidative damage. The aim of the study is to investigate neuroprotective properties of Coptis chinensis Franch. rhizome watery extract (CRE) and to evaluate its potential mechanism of action.
Neuroprotective properties on t-BOOH induced oxidative stress were investigated in SH-SY5Y human neuroblastoma cells. Cells were pretreated with CRE for 2 h or 24 h followed by 2 h of treatment with t-BOOH. To evaluate the neuroprotective effect of CRE, cell viability, cellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP) and the apoptotic rate were determined and microarray analyses, as well as qRT-PCR analyses were conducted.
Two hours of exposure to 100 µM t-BOOH resulted in a significant reduction of cell viability, increased apoptotic rate, declined mitochondrial membrane potential (MMP) and increased ROS production. Reduction of cell viability, increased apoptotic rate and declined mitochondrial membrane potential (MMP) could be significantly reduced in cells pretreated with CRE (100 µg/ml) for 2h or 24h ahead of t-BOOH exposure with the greatest effect after 24h of pretreatment; however ROS production was not changed significantly. Furthermore, microarray analyses revealed that the expressions of 2 genes; thioredoxin-interacting protein (TXNIP) and mitochondrially encoded NADH dehydrogenase 1, were significantly regulated. Down regulation of TXNIP was confirmed by qRT-PCR.
Due to its neuroprotective properties CRE might be a potential therapeutic agent for the prevention or amelioration of diseases like diabetic neuropathy and neurodegenerative disorders like Alzheimer and Parkinsons disease.
黄连（毛茛科）干燥根茎在传统中药中用于治疗炎症性疾病和糖尿病。最近的研究表明黄连生物碱具有多种活性，包括神经保护、神经再生、抗糖尿病、抗氧化和抗炎作用。然而，尚无关于黄连水提取物对叔丁基过氧化氢（t-BOOH）诱导的氧化损伤的神经保护作用的报道。本研究的目的是研究黄连根茎水提取物（CRE）的神经保护特性并评估其潜在作用机制。
在SH-SY5Y人神经母细胞瘤细胞中研究了对t-BOOH诱导的氧化应激的神经保护特性。细胞先用CRE预处理2小时或24小时，然后用t-BOOH处理2小时。为评估CRE的神经保护作用，测定了细胞活力、细胞活性氧（ROS）、线粒体膜电位（MMP）和凋亡率，并进行了微阵列分析以及qRT-PCR分析。
暴露于100μM t-BOOH 2小时导致细胞活力显著降低、凋亡率增加、线粒体膜电位（MMP）下降和ROS产生增加。在用CRE（100μg/ml）预处理2小时或24小时后再暴露于t-BOOH的细胞中，细胞活力降低、凋亡率增加和线粒体膜电位（MMP）下降可显著降低，预处理24小时后效果最佳；然而ROS产生没有显著变化。此外，微阵列分析显示2个基因的表达；硫氧还蛋白相互作用蛋白（TXNIP）和线粒体编码的NADH脱氢酶1，受到显著调节。qRT-PCR证实了TXNIP的下调。
由于其神经保护特性，CRE可能是预防或改善糖尿病性神经病变以及阿尔茨海默病和帕金森病等神经退行性疾病的潜在治疗剂。
