Villalobos Alvaro S, Wiese Jutta, Aguilar Pablo, Dorador Cristina, Imhoff Johannes F
Marine Microbiology, GEOMAR Helmholtz Centre for Ocean Research Kiel, Düsternbrooker Weg 20, 24105, Kiel, Germany.
Laboratorio de Complejidad Microbiana y Ecología Funcional and Departamento de Biotecnología, Facultad de Ciencias del Mar y Recursos Biológicos, Universidad de Antofagasta, Antofagasta, Chile.
Antonie Van Leeuwenhoek. 2018 Jun;111(6):955-963. doi: 10.1007/s10482-017-0994-4. Epub 2017 Dec 6.
A novel actinobacterium, strain DB165, was isolated from cold waters of Llullaillaco Volcano Lake (6170 m asl) in Chile. Phylogenetic analysis based on 16S rRNA gene sequences identified strain DB165 as belonging to the genus Subtercola in the family Microbacteriaceae, sharing 97.4% of sequence similarity with Subtercola frigoramans DSM 13057, 96.7% with Subtercola lobariae DSM 103962, and 96.1% with Subtercola boreus DSM 13056. The cells were observed to be Gram-positive, form rods with irregular morphology, and to grow best at 10-15 °C, pH 7 and in the absence of NaCl. The cross-linkage between the amino acids in its peptidoglycan is type B2γ; 2,4-diaminobutyric acid is the diagnostic diamino acid; the major respiratory quinones are MK-9 and MK-10; and the polar lipids consist of phosphatidylglycerol, diphosphatidylglycerol, 5 glycolipids, 2 phospholipids and 5 additional polar lipids. The fatty acid profile of DB165 (5% >) contains iso-C14:0, iso-C16:0, anteiso-C15:0, anteiso-C17:0, and the dimethylacetal iso-C16:0 DMA. The genomic DNA G+C content of strain DB165 was determined to be 65 mol%. Based on the phylogenetic, phenotypic, and chemotaxonomic analyses presented in this study, strain DB165 (= DSM 105013 = JCM 32044) represents a new species in the genus Subtercola, for which the name Subtercola vilae sp. nov. is proposed.
从智利尤耶亚科火山湖（海拔6170米）的冷水中分离出一种新型放线菌，菌株DB165。基于16S rRNA基因序列的系统发育分析确定菌株DB165属于微杆菌科地下杆菌属，与嗜冷地下杆菌DSM 13057的序列相似性为97.4%，与地衣地下杆菌DSM 103962的序列相似性为96.7%，与北方地下杆菌DSM 13056的序列相似性为96.1%。观察到该菌细胞为革兰氏阳性，形态不规则呈杆状，在10-15℃、pH 7且无氯化钠的条件下生长最佳。其肽聚糖中氨基酸之间的交联类型为B2γ；2,4-二氨基丁酸是诊断性二氨基酸；主要呼吸醌为MK-9和MK-10；极性脂质由磷脂酰甘油、双磷脂酰甘油、5种糖脂、2种磷脂和5种其他极性脂质组成。DB165的脂肪酸谱（>5%）包含异C14:0、异C16:0、anteiso-C15:0、anteiso-C17:0以及二甲基缩醛异C16:0 DMA。菌株DB165的基因组DNA G+C含量测定为65 mol%。基于本研究中呈现的系统发育、表型和化学分类分析，菌株DB165（=DSM 105013 =JCM 32044）代表地下杆菌属中的一个新物种，为此提出名为维拉地下杆菌新种（Subtercola vilae sp. nov.）。
