Tatar Demet, Guven Kiymet, Inan Kadriye, Cetin Demet, Belduz Ali Osman, Sahin Nevzat
Biology Department, Faculty of Art and Science, Ondokuz Mayis University, 55139, Atakum-Samsun, Turkey.
Biology Department, Faculty of Science, Anadolu University, 26470, Eskisehir, Turkey.
Antonie Van Leeuwenhoek. 2016 Jan;109(1):35-41. doi: 10.1007/s10482-015-0607-z. Epub 2015 Oct 12.
A novel actinobacterium, designated strain BN506(T), was isolated from soil collected from Tuz (Salt) Lake, Konya, Turkey, and was characterised to determine its taxonomic position. The isolate was found to have morphological and chemotaxonomic properties associated with members of the genus Streptomonospora. The isolate was found to grow optimally at 37 °C and in the presence of 10 % (w/v) NaCl but not in the absence of NaCl. Phylogenetic analyses based on an almost-complete 16S rRNA gene sequences indicated that isolate is closely related to members of the genus Streptomonospora and forms a distinct phyletic line in the Streptomonospora phylogenetic tree. Strain BN506(T) is closely related to Streptomonospora halophila YIM 91355(T) (98.1 % sequence similarity). Sequence similarities with other type strains of the genus Streptomyces were lower than 98.0 %. The cell wall of the novel strain was found to contain meso-diaminopimelic acid. Whole cell hydrolysates were found to contain galactose, glucose and ribose. The predominant menaquinone was identified as MK-10(H8) (57.0 %). The polar lipids detected were identified as diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine. The major fatty acids were found to be anteiso-C17:0, iso-C16:0 and 10 methyl C18:0. Based on 16S rRNA gene sequence analysis, DNA-DNA relatedness, phenotypic characteristics and chemotaxonomic data, strain BN506(T) was identified as a member of a novel species of the genus Streptomonospora, for which the name Streptomonospora tuzyakensis sp. nov. (type strain BN506(T) = DSM 45930(T) = KCTC 29210(T)) is proposed.
从土耳其科尼亚图兹（盐）湖采集的土壤中分离出一株新型放线菌，命名为菌株BN506(T)，并对其进行了鉴定以确定其分类地位。该分离株具有与链单孢菌属成员相关的形态和化学分类学特性。发现该分离株在37℃和10%（w/v）NaCl存在的条件下生长最佳，但在无NaCl的情况下不生长。基于近乎完整的16S rRNA基因序列的系统发育分析表明，该分离株与链单孢菌属成员密切相关，并在链单孢菌系统发育树中形成一个独特的进化分支。菌株BN506(T)与嗜盐链单孢菌YIM 91355(T)密切相关（序列相似性为98.1%）。与链霉菌属其他模式菌株的序列相似性低于98.0%。发现该新型菌株的细胞壁含有内消旋二氨基庚二酸。全细胞水解产物含有半乳糖、葡萄糖和核糖。主要的甲基萘醌被鉴定为MK-10(H8)（57.0%）。检测到的极性脂质被鉴定为双磷脂酰甘油、磷脂酰甘油和磷脂酰胆碱。主要脂肪酸为anteiso-C17:0、iso-C16:0和10-甲基C18:0。基于16S rRNA基因序列分析、DNA-DNA相关性、表型特征和化学分类学数据，菌株BN506(T)被鉴定为链单孢菌属一个新物种的成员，为此提出了新种名嗜图兹湖链单孢菌（Streptomonospora tuzyakensis sp. nov.）（模式菌株BN506(T) = DSM 45930(T) = KCTC 29210(T)）。
