Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, The Netherlands.
Research Area Biochemical Technology, Institute of Chemical, Environmental and Biological Engineering, TU Wien, Vienna, Austria.
FEBS Lett. 2018 Jan;592(1):60-70. doi: 10.1002/1873-3468.12932. Epub 2017 Dec 19.
Trichoderma reesei is used to produce saccharifying enzyme cocktails for biofuels. There is limited understanding of the transcription factors (TFs) that regulate genes involved in release and catabolism of l-arabinose and d-galactose, as the main TF XYR1 is only partially involved. Here, the T. reesei ortholog of ARA1 from Pyricularia oryzae that regulates l-arabinose releasing and catabolic genes was deleted and characterized by growth profiling and transcriptomics along with a xyr1 mutant and xyr1/ara1 double mutant. Our results show that in addition to the l-arabinose-related role, T. reesei ARA1 is essential for expression of d-galactose releasing and catabolic genes, while XYR1 is not involved in this process.
里氏木霉被用于生产糖化酶混合物以用于生物燃料。对于调节释放和分解 l-阿拉伯糖和 d-半乳糖的基因的转录因子（TFs）的了解有限，因为主要的 TF XYR1 只是部分参与。在这里，我们删除了水稻纹枯病菌中调节 l-阿拉伯糖释放和分解基因的 ARA1 的里氏木霉直系同源物，并通过生长分析和转录组学以及 xyr1 突变体和 xyr1/ara1 双突变体进行了表征。我们的结果表明，除了与 l-阿拉伯糖相关的作用外，木霉 ARA1 对于 d-半乳糖释放和分解基因的表达是必需的，而 XYR1 不参与此过程。
