Jia Jianguo, Zhang Yang, Zheng Min, Shan Changfu, Yan Huicheng, Wu Wenyu, Gao Xuan, Cheng Bo, Liu Weisheng, Tang Yu
State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, and ‡Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University , Lanzhou 730000, P. R. China.
Inorg Chem. 2018 Jan 2;57(1):300-310. doi: 10.1021/acs.inorgchem.7b02475. Epub 2017 Dec 8.
The postsynthetic-modified nanoscale metal-organic framework (NMOF) probes selected as potential drug delivery platforms and photodynamic therapy agents to fulfill the effective and safe treatment of neoplastic diseases have attracted increasing attention recently. Herein, a Eu(III)-based NMOF probe elaborately postsynthetically modified with a β-diketonate two-photon-absorbing (TPA) ligand is rationally designed and further functionalized by assembling the photosensitizer molecule (methylene blue, MB) in the pores and a cyclic peptide targeting motif on the surface of the NMOF, which could achieve highly efficient near-infrared (NIR)-triggered and -targeted photodynamic therapy (PDT). On the basis of the luminescence resonance energy transfer process between the NMOF donor and the photosensitizer MB acceptor, the probe can achieve a high tissue-penetrable TPA-PDT effect. Thus, the NMOFs in this study play the role of not only the nanocontainer for the photosensitizer but also the energy-transfer donor. Studies in vitro show enhanced cellular uptake and satisfactory PDT effectiveness toward cancer cells compared to the free photosensitizer MB. It is highly expected that this study contributes to the development of smart luminescent diagnostic and therapeutic probes.
作为潜在的药物递送平台和光动力治疗剂以实现对肿瘤疾病的有效和安全治疗而被选择的合成后修饰的纳米级金属有机框架（NMOF）探针最近受到了越来越多的关注。在此，合理设计了一种基于铕（III）的NMOF探针，该探针通过β-二酮双光子吸收（TPA）配体进行了精心的合成后修饰，并通过在NMOF的孔中组装光敏剂分子（亚甲蓝，MB）和在其表面上的环状肽靶向基序进一步功能化，这可以实现高效的近红外（NIR）触发和靶向光动力治疗（PDT）。基于NMOF供体和光敏剂MB受体之间的发光共振能量转移过程，该探针可以实现高组织穿透性的TPA-PDT效应。因此，本研究中的NMOF不仅起到了光敏剂纳米容器的作用，还起到了能量转移供体的作用。体外研究表明，与游离光敏剂MB相比，对癌细胞的细胞摄取增强且PDT效果令人满意。高度期望本研究有助于智能发光诊断和治疗探针的开发。
