Tumor-suppressive function of in papillary thyroid cancer.

BACKGROUND

Studies have shown an association of the gene with kidney and bladder cancer and neuroblastoma. We investigated whether acts as a tumor suppressor in papillary thyroid carcinoma (PTC).

METHODS

Primary PTC tumors and matched normal thyroid tissues were obtained from 112 patients to detect mRNA by real-time PCR. Genomic DNA sequencing was performed to detect mutation in PTC tumors. The association between expression and clinicopathological data from PTC patients was reviewed retrospectively. PTC-derived cancer cell lines TPC-1 and K1 with stable transfection of were used to investigate the functions of . Flow cytometry, CCK-8, Transwell assay and scratch tests were used to examine cell cycle distribution, proliferation and migration.

RESULTS

The expression of was significantly decreased in PTC compared with adjacent normal thyroid tissues. Lower expression was significantly associated with aggressive tumor behaviors, such as lymph node metastasis and mutation. Overexpression of significantly suppressed malignant cell behaviors, including cell proliferation and migration, as well as tumor growth .

CONCLUSIONS

These findings suggest a potential tumor suppressor role of in PTC progression; and provide insight into potential clinical relevance for the prognosis of PTC.