Zhang Man-Man, Sun Feng, Cui Bing, Zhang Le-Le, Fang Ya, Li Yan, Zhang Rui-Jia, Ye Xiao-Ping, Ma Yu-Ru, Han Bing, Song Huai-Dong
The Core Laboratory in Medicine Center of Clinical Research, Department of Endocrinology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China.
Department of Transfusion, The Hospital Affiliated to Jiangsu University, Zhenjiang 212001, China.
Oncotarget. 2017 Oct 10;8(56):96126-96138. doi: 10.18632/oncotarget.21759. eCollection 2017 Nov 10.
Studies have shown an association of the gene with kidney and bladder cancer and neuroblastoma. We investigated whether acts as a tumor suppressor in papillary thyroid carcinoma (PTC).
Primary PTC tumors and matched normal thyroid tissues were obtained from 112 patients to detect mRNA by real-time PCR. Genomic DNA sequencing was performed to detect mutation in PTC tumors. The association between expression and clinicopathological data from PTC patients was reviewed retrospectively. PTC-derived cancer cell lines TPC-1 and K1 with stable transfection of were used to investigate the functions of . Flow cytometry, CCK-8, Transwell assay and scratch tests were used to examine cell cycle distribution, proliferation and migration.
The expression of was significantly decreased in PTC compared with adjacent normal thyroid tissues. Lower expression was significantly associated with aggressive tumor behaviors, such as lymph node metastasis and mutation. Overexpression of significantly suppressed malignant cell behaviors, including cell proliferation and migration, as well as tumor growth .
These findings suggest a potential tumor suppressor role of in PTC progression; and provide insight into potential clinical relevance for the prognosis of PTC.
研究表明该基因与肾癌、膀胱癌和神经母细胞瘤有关联。我们研究了其在甲状腺乳头状癌（PTC）中是否作为一种肿瘤抑制因子发挥作用。
从112例患者获取原发性PTC肿瘤组织及配对的正常甲状腺组织，通过实时PCR检测其mRNA。对PTC肿瘤进行基因组DNA测序以检测其突变情况。回顾性分析PTC患者中该基因表达与临床病理数据之间的关联。使用稳定转染该基因的PTC来源的癌细胞系TPC-1和K1来研究其功能。采用流式细胞术、CCK-8、Transwell实验和划痕实验检测细胞周期分布、增殖和迁移情况。
与相邻正常甲状腺组织相比，PTC中该基因的表达显著降低。较低的该基因表达与侵袭性肿瘤行为显著相关，如淋巴结转移和基因突变。该基因的过表达显著抑制恶性细胞行为，包括细胞增殖和迁移以及肿瘤生长。
这些发现提示该基因在PTC进展中具有潜在的肿瘤抑制作用；并为PTC预后的潜在临床相关性提供了见解。
