College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Int J Biol Macromol. 2019 Jun 1;130:399-406. doi: 10.1016/j.ijbiomac.2019.02.150. Epub 2019 Feb 26.
The lectin from Latiporus sulphureus (LSL) was purified by chromatography. Five components were obtained, of which LSL4 was the most effective one to promote cell growth, which increased cell viability to 2.6 times and 0.8 times of negative control and positive control group, so LSL4 with the highest immunomodulatory activity was selected for further identification. In addition, its immune effects on murine macrophages in vitro were evaluated. After LSL4 treatment, cell viability and phagocytic activity were greatly improved, and NO amount could reach about 24.795 ± 1.559 μmol·L. The contents of TNF-α and IL-10 were 4.0 and 3.2 times higher than those of the negative control group, and the contents of IL-1β and IL-6 were 2.8 and 4.1 times higher than those of the negative control group, respectively. TAK242 significantly inhibited the secretion of TNF-α, IL-1β, IL-6 and IL-10, with inhibition rates of 53%, 61%, 48% and 54%, respectively. The results showed that 76.0 kD glycoprotein could promote cell proliferation and phagocytosis, and activate cells to release more nitric oxide, nitric oxide synthase and cytokines. The lectin has potential immunopotentiation in functional foods and pharmacology.
硫色多孔菌（LSL）中的凝集素通过色谱法进行了纯化。得到了五个组分，其中 LSL4 是最有效的促进细胞生长的组分，可使细胞活力增加到阴性对照组和阳性对照组的 2.6 倍和 0.8 倍，因此选择具有最高免疫调节活性的 LSL4 进行进一步鉴定。此外，还评估了其对体外鼠巨噬细胞的免疫作用。LSL4 处理后，细胞活力和吞噬活性大大提高，NO 量可达到约 24.795±1.559µmol·L。TNF-α 和 IL-10 的含量分别比阴性对照组高 4.0 倍和 3.2 倍，IL-1β 和 IL-6 的含量分别比阴性对照组高 2.8 倍和 4.1 倍。TAK242 显著抑制 TNF-α、IL-1β、IL-6 和 IL-10 的分泌，抑制率分别为 53%、61%、48%和 54%。结果表明，76.0kD 糖蛋白可促进细胞增殖和吞噬作用，并激活细胞释放更多的一氧化氮、一氧化氮合酶和细胞因子。该凝集素在功能性食品和药理学中具有潜在的免疫增强作用。
