College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Int J Biol Macromol. 2019 Jan;121:846-851. doi: 10.1016/j.ijbiomac.2018.10.080. Epub 2018 Oct 17.
Umbilicaria esculenta possesses nutritional properties, such as antioxidant, antithrombotic and lipid peroxidation inhibitory activity. The immune effects of its polysaccharide (Umbilicaria esculenta polysaccharide, UEP) on murine macrophages RAW264.7 were investigated for the first time. UEP promoted their proliferation and phagocytic activity obviously. At the concentration of 600 μg mL, UEP stimulated their proliferation and phagocytosis to 1.4 and 2.5 times, respectively, as compared with the negative group. Moreover, UEP induced their release of nitric oxide (NO), NO synthase (NOS), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ) and interleukin factors (IL-1β, IL-6 and IL-10) in a concentration-dependent manner. The production of NO and NOS was increased to 25.2 and 2.7 times, respectively, while the secretion of those cytokines was enhanced to multiples from 1.9 to 2.6 times. At the same time, TAK242, an inhibitor of TLR4 (Toll-like receptor 4), inhibited UEP influences on these factors with inhibition rates up to 50%, which indicated that UEP acted on murine macrophages mainly via TLR4. UEP has positive immunomodulatory functions in murine macrophages, and can be developed as a potential novel immunomodulator.
皱边网褶菌具有抗氧化、抗血栓和抑制脂质过氧化等营养特性。本研究首次探讨了皱边网褶菌多糖（Umbilicaria esculenta polysaccharide，UEP）对 RAW264.7 巨噬细胞的免疫作用。UEP 能明显促进 RAW264.7 细胞的增殖和吞噬活性，当浓度为 600μg/mL 时，UEP 对细胞的增殖和吞噬作用分别增强至 1.4 倍和 2.5 倍。UEP 还能呈浓度依赖性诱导巨噬细胞释放一氧化氮（NO）、NO 合酶（NOS）、肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）和白细胞介素因子（IL-1β、IL-6 和 IL-10）。NO 和 NOS 的产生分别增加至 25.2 倍和 2.7 倍，而这些细胞因子的分泌则增强至 1.9 至 2.6 倍。TLR4（Toll-like receptor 4）抑制剂 TAK242 可抑制 UEP 对这些因子的影响，抑制率高达 50%，这表明 UEP 主要通过 TLR4 作用于巨噬细胞。UEP 对巨噬细胞具有积极的免疫调节功能，可作为一种有潜力的新型免疫调节剂。
